ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNDETVEKV TQQVSELKST DVKEQVVTPW DVEGGVDEQG RAQNIDYDKL IKQFGTKPVN 

        70         80         90        100        110        120 
EETLKRFKQV TGREPHHFLR KGLFFSERDF TKILDLYEQG KPFFLYTGRG PSSDSMHLGH 

       130        140        150        160        170        180 
MIPFVFTKWL QEVFDVPLVI ELTDDEKFLF KHKLTINDVK NFARENAKDI IAVGFDPKNT 

       190        200        210        220        230        240 
FIFSDLQYMG GAFYETVVRV SRQITGSTAK AVFGFNDSDC IGKFHFASIQ IATAFPSSFP 

       250        260        270        280        290        300 
NVLGLPDKTP CLIPCAIDQD PYFRVCRDVA DKLKYSKPAL LHSRFFPALQ GSTTKMSASD 

       310        320        330        340        350        360 
DTTAIFMTDT PKQIQKKINK YAFSGGQVSA DLHRELGGNP DVDVAYQYLS FFKDDDVFLK 

       370        380        390        400        410        420 
ECYDKYKSGE LLSGEMKKLC IETLQEFVKA FQERRAQVDE ETLDKFMVPH KLVWGEKERL 

       430 
VAPKPKTKQE KK 

References and documentation are available.

Number of amino acids: 432

Molecular weight: 49350.33

Theoretical pI: 6.35

Amino acid composition: 
Ala (A)  22	  5.1%
Arg (R)  16	  3.7%
Asn (N)  11	  2.5%
Asp (D)  35	  8.1%
Cys (C)   6	  1.4%
Gln (Q)  22	  5.1%
Glu (E)  28	  6.5%
Gly (G)  25	  5.8%
His (H)   9	  2.1%
Ile (I)  19	  4.4%
Leu (L)  33	  7.6%
Lys (K)  44	 10.2%
Met (M)   8	  1.9%
Phe (F)  35	  8.1%
Pro (P)  21	  4.9%
Ser (S)  24	  5.6%
Thr (T)  27	  6.2%
Trp (W)   3	  0.7%
Tyr (Y)  12	  2.8%
Val (V)  32	  7.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      2235
Hydrogen    H	      3454
Nitrogen    N	       578
Oxygen      O	       655
Sulfur      S	        14

Formula: C2235H3454N578O655S14
Total number of atoms: 6936

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34755
Abs 0.1% (=1 g/l)   0.704, assuming all pairs of Cys residues form cystines


Ext. coefficient    34380
Abs 0.1% (=1 g/l)   0.697, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.88
This classifies the protein as stable.



Aliphatic index: 73.52

Grand average of hydropathicity (GRAVY): -0.452