ProtParam
User-provided sequence:
10 20 30 40 50 60
MSNDETVEKV TQQVSELKST DVKEQVVTPW DVEGGVDEQG RAQNIDYDKL IKQFGTKPVN
70 80 90 100 110 120
EETLKRFKQV TGREPHHFLR KGLFFSERDF TKILDLYEQG KPFFLYTGRG PSSDSMHLGH
130 140 150 160 170 180
MIPFVFTKWL QEVFDVPLVI ELTDDEKFLF KHKLTINDVK NFARENAKDI IAVGFDPKNT
190 200 210 220 230 240
FIFSDLQYMG GAFYETVVRV SRQITGSTAK AVFGFNDSDC IGKFHFASIQ IATAFPSSFP
250 260 270 280 290 300
NVLGLPDKTP CLIPCAIDQD PYFRVCRDVA DKLKYSKPAL LHSRFFPALQ GSTTKMSASD
310 320 330 340 350 360
DTTAIFMTDT PKQIQKKINK YAFSGGQVSA DLHRELGGNP DVDVAYQYLS FFKDDDVFLK
370 380 390 400 410 420
ECYDKYKSGE LLSGEMKKLC IETLQEFVKA FQERRAQVDE ETLDKFMVPH KLVWGEKERL
430
VAPKPKTKQE KK
References and
documentation are available.
Number of amino acids: 432
Molecular weight: 49350.33
Theoretical pI: 6.35
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 60
Atomic composition:
Carbon C 2235
Hydrogen H 3454
Nitrogen N 578
Oxygen O 655
Sulfur S 14
Formula: C
2235H
3454N
578O
655S
14
Total number of atoms: 6936
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34755
Abs 0.1% (=1 g/l) 0.704, assuming all pairs of Cys residues form cystines
Ext. coefficient 34380
Abs 0.1% (=1 g/l) 0.697, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.88
This classifies the protein as stable.
Aliphatic index: 73.52
Grand average of hydropathicity (GRAVY): -0.452