ProtParam
User-provided sequence:
10 20 30 40 50 60
MSNDQLKSKL REFFDRQPDQ SEKAHQDSKI FEVLYSQFLE NQHCIDWNSW KFLEEKHQVT
70 80 90 100 110 120
LKDLEPFDKS RFNILNKLAV IKLNGGLGTT MGCSKAKSLV EVREGYTFMD LAVLEHQKMC
130 140 150 160 170 180
EAHNVDTPLY LMNSFYTDED TKKYLAEKGY SNVKTFVQSK CPRLDAETKL PIEDENEDWG
190 200 210 220 230 240
DDAWCPPGHG NIFQSLQNSG VLDQLLADGR EIIFVSNIDN TGANTDLQIV QLMLDKNVDY
250 260 270 280 290 300
IMECTPKTQV DVKGGTLIDI GGRMMHLEMP QVPAENLPDF CSTKVFKIFN TNNIYVNLKA
310 320 330 340 350 360
VKKLLPDIKS EIIVNKKTIR SREVLQLEFS IGGCIKNFDN ALCVHVERKR FRPVKNLGDL
370 380 390 400 410 420
LSLRSTLCDL DHSTFKVHHN HELGAPPVIS LDPSIYNSVE VVDLKFPHPL VMDNCSEFAV
430 440 450 460
VGDVTFGKNV KLSGKVTVNG KTESPGVVPD GTVLKDQEYI AE
References and
documentation are available.
Number of amino acids: 462
Molecular weight: 52252.67
Theoretical pI: 5.47
Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 53
Atomic composition:
Carbon C 2322
Hydrogen H 3663
Nitrogen N 619
Oxygen O 706
Sulfur S 22
Formula: C
2322H
3663N
619O
706S
22
Total number of atoms: 7332
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 37525
Abs 0.1% (=1 g/l) 0.718, assuming all pairs of Cys residues form cystines
Ext. coefficient 36900
Abs 0.1% (=1 g/l) 0.706, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.22
This classifies the protein as stable.
Aliphatic index: 87.88
Grand average of hydropathicity (GRAVY): -0.361