ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNDQLKSKL REFFDRQPDQ SEKAHQDSKI FEVLYSQFLE NQHCIDWNSW KFLEEKHQVT 

        70         80         90        100        110        120 
LKDLEPFDKS RFNILNKLAV IKLNGGLGTT MGCSKAKSLV EVREGYTFMD LAVLEHQKMC 

       130        140        150        160        170        180 
EAHNVDTPLY LMNSFYTDED TKKYLAEKGY SNVKTFVQSK CPRLDAETKL PIEDENEDWG 

       190        200        210        220        230        240 
DDAWCPPGHG NIFQSLQNSG VLDQLLADGR EIIFVSNIDN TGANTDLQIV QLMLDKNVDY 

       250        260        270        280        290        300 
IMECTPKTQV DVKGGTLIDI GGRMMHLEMP QVPAENLPDF CSTKVFKIFN TNNIYVNLKA 

       310        320        330        340        350        360 
VKKLLPDIKS EIIVNKKTIR SREVLQLEFS IGGCIKNFDN ALCVHVERKR FRPVKNLGDL 

       370        380        390        400        410        420 
LSLRSTLCDL DHSTFKVHHN HELGAPPVIS LDPSIYNSVE VVDLKFPHPL VMDNCSEFAV 

       430        440        450        460 
VGDVTFGKNV KLSGKVTVNG KTESPGVVPD GTVLKDQEYI AE 

References and documentation are available.

Number of amino acids: 462

Molecular weight: 52252.67

Theoretical pI: 5.47

Amino acid composition: 
Ala (A)  16	  3.5%
Arg (R)  13	  2.8%
Asn (N)  30	  6.5%
Asp (D)  36	  7.8%
Cys (C)  11	  2.4%
Gln (Q)  18	  3.9%
Glu (E)  31	  6.7%
Gly (G)  26	  5.6%
His (H)  13	  2.8%
Ile (I)  24	  5.2%
Leu (L)  47	 10.2%
Lys (K)  40	  8.7%
Met (M)  11	  2.4%
Phe (F)  22	  4.8%
Pro (P)  20	  4.3%
Ser (S)  28	  6.1%
Thr (T)  23	  5.0%
Trp (W)   4	  0.9%
Tyr (Y)  10	  2.2%
Val (V)  39	  8.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2322
Hydrogen    H	      3663
Nitrogen    N	       619
Oxygen      O	       706
Sulfur      S	        22

Formula: C2322H3663N619O706S22
Total number of atoms: 7332

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37525
Abs 0.1% (=1 g/l)   0.718, assuming all pairs of Cys residues form cystines


Ext. coefficient    36900
Abs 0.1% (=1 g/l)   0.706, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.22
This classifies the protein as stable.



Aliphatic index: 87.88

Grand average of hydropathicity (GRAVY): -0.361