ProtParam
User-provided sequence:
10 20 30 40 50 60
MSNEAYQHDH TVNPHQKIVV NSYDWLQFRD EQDHCKSKNP ITHASPGVGS NAQNSDIAEA
70 80 90 100 110 120
PQVFHPSYQS LVNVPSESPR PDQTSGSNPA VGLLHNAEDK ASGQEEEGSQ YEIQYSVFRP
130 140 150 160 170 180
LHAYPTKGLA YEQLRRKEEQ EQRENFNHLV SDCIEAVETF GRELERIQTG SSGSYFVYGT
190 200 210 220 230 240
RADESVPVGV FKPKDEEPYG PFSPKWTKWA HRTFFPCLFG RSCLIPNLGY ICESAASLLD
250 260 270 280 290 300
RRLETHLVPY TDTASIESFN FYDNRKKWVL GYNLQKKKQK KLGSFQLFLK EYINADEFFH
310 320 330 340 350 360
KYPLPGMYSD VKHSFHRKSS GEDINHKPET TRNLTDETEP SKQINSSPIS TESEENSKFE
370 380 390 400 410 420
WTESSLSQFR LELEKLIILD YIMRNTDRGL DNWMVKLIKL SNNKWRLKLA AIDNGLSFPW
430 440 450 460 470 480
KHPDEWRLYP YGWLYLPLQL LAKPFSEQMR SHFLPILTST NWWEESYQEF LALFSRDQDF
490 500 510 520 530 540
NVRMWKKQWA VLKGQAFNVV ETLKDPRQGP LELVRRTRCQ VIDEKMQVPC CPPPVSIFKN
550 560 570 580 590 600
AIDEPIGSYS TSPMVLPSTP STIPFHAHNQ SNSNPVYYDS TLHPFANKTV IAERLQIVNS
TPVFTWC
References and
documentation are available.
Number of amino acids: 607
Molecular weight: 70216.93
Theoretical pI: 6.23
Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 67
Atomic composition:
Carbon C 3160
Hydrogen H 4786
Nitrogen N 852
Oxygen O 935
Sulfur S 17
Formula: C
3160H
4786N
852O
935S
17
Total number of atoms: 9750
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 120250
Abs 0.1% (=1 g/l) 1.713, assuming all pairs of Cys residues form cystines
Ext. coefficient 119750
Abs 0.1% (=1 g/l) 1.705, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.79
This classifies the protein as unstable.
Aliphatic index: 69.21
Grand average of hydropathicity (GRAVY): -0.671