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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNEIKTQVV VLGAGPAGYS AAFRAADLGL ETVIVERFST LGGVCLNVGC IPSKALLHVA

70 80 90 100 110 120
KVIEEAKAVA AHGVVFGEPT IDLDKLRSFK QKVISQLTGG LGGMSKMRKV NVVNGFGKFS

130 140 150 160 170 180
GPNSLEVTAE DGTVTVVKFD QAIIAAGSRP IKLPFIPHED PRIWDSTDAL ELKEVPGKLL

190 200 210 220 230 240
VMGGGIIGLE MGTVYSSLGS EIDVVEMFDQ VIPAADKDVV RVFTKQIKKK FNLILETKVT

250 260 270 280 290 300
AVEAREDGIY VSMEGKSAPA EPVRYDAVLV AIGRTPNGKL IDAEKAGVKI DERGFINVDK

310 320 330 340 350 360
QLRTNVPHIY AIGDIVGQPM LAHKGVHEGH VAAEVIAGMK HYFDPKVIPS IAYTDPEVAW

370 380 390 400 410 420
VGLTEKEAKE QGIAYETATF PWAASGRAIA SDCSEGMTKL IFDKDTHRVI GGAIVGVNGG

430 440 450 460 470
ELLGEIGLAI EMGCDAEDLA LTIHAHPTLH ESVGLAAEIY EGSITDLPNP KAKKK


References and documentation are available.

Number of amino acids: 475 Molecular weight: 50536.33 Theoretical pI: 5.56
Amino acid composition: 
Ala (A) 49 10.3% Arg (R) 14 2.9% Asn (N) 11 2.3% Asp (D) 26 5.5% Cys (C) 4 0.8% Gln (Q) 9 1.9% Glu (E) 36 7.6% Gly (G) 50 10.5% His (H) 12 2.5% Ile (I) 38 8.0% Leu (L) 35 7.4% Lys (K) 36 7.6% Met (M) 11 2.3% Phe (F) 15 3.2% Pro (P) 22 4.6% Ser (S) 22 4.6% Thr (T) 24 5.1% Trp (W) 3 0.6% Tyr (Y) 9 1.9% Val (V) 49 10.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 62 Total number of positively charged residues (Arg + Lys): 50 Atomic composition: Carbon C 2263 Hydrogen H 3642 Nitrogen N 600 Oxygen O 675 Sulfur S 15 Formula: C2263H3642N600O675S15 Total number of atoms: 7195 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 30160 Abs 0.1% (=1 g/l) 0.597, assuming all pairs of Cys residues form cystines Ext. coefficient 29910 Abs 0.1% (=1 g/l) 0.592, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 25.66 This classifies the protein as stable. Aliphatic index: 100.17 Grand average of hydropathicity (GRAVY): 0.080