ProtParam
User-provided sequence:
10 20 30 40 50 60
MSNEIKTQVV VLGAGPAGYS AAFRAADLGL ETVIVERFST LGGVCLNVGC IPSKALLHVA
70 80 90 100 110 120
KVIEEAKAVA AHGVVFGEPT IDLDKLRSFK QKVISQLTGG LGGMSKMRKV NVVNGFGKFS
130 140 150 160 170 180
GPNSLEVTAE DGTVTVVKFD QAIIAAGSRP IKLPFIPHED PRIWDSTDAL ELKEVPGKLL
190 200 210 220 230 240
VMGGGIIGLE MGTVYSSLGS EIDVVEMFDQ VIPAADKDVV RVFTKQIKKK FNLILETKVT
250 260 270 280 290 300
AVEAREDGIY VSMEGKSAPA EPVRYDAVLV AIGRTPNGKL IDAEKAGVKI DERGFINVDK
310 320 330 340 350 360
QLRTNVPHIY AIGDIVGQPM LAHKGVHEGH VAAEVIAGMK HYFDPKVIPS IAYTDPEVAW
370 380 390 400 410 420
VGLTEKEAKE QGIAYETATF PWAASGRAIA SDCSEGMTKL IFDKDTHRVI GGAIVGVNGG
430 440 450 460 470
ELLGEIGLAI EMGCDAEDLA LTIHAHPTLH ESVGLAAEIY EGSITDLPNP KAKKK
References and
documentation are available.
Number of amino acids: 475
Molecular weight: 50536.33
Theoretical pI: 5.56
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 2263
Hydrogen H 3642
Nitrogen N 600
Oxygen O 675
Sulfur S 15
Formula: C
2263H
3642N
600O
675S
15
Total number of atoms: 7195
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30160
Abs 0.1% (=1 g/l) 0.597, assuming all pairs of Cys residues form cystines
Ext. coefficient 29910
Abs 0.1% (=1 g/l) 0.592, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 25.66
This classifies the protein as stable.
Aliphatic index: 100.17
Grand average of hydropathicity (GRAVY): 0.080