ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNFDDLQIL DQLQTSARFR GDTRIRRFKG SNNSKSPFGL NPSSRISQWP RITFIRAKDN 

        70         80         90        100        110        120 
YNPAEELCQL IDEQNAHNEA KHQSKEIMLP KETNVKSCED LSKTGEIKAL DIVLFKAITS 

       130        140        150        160        170        180 
KGDTLKVKKI PEYAEAFDTT KNVEVSSFPE KSMNENVLIQ HSLNLAPSDK EISSTEESEQ 

       190        200        210        220        230        240 
LCYKEQESEK ELYSKDNDDS LDILNVPNLV NDDIANNNAA PPPPLAQAQE QLSTENEDEF 

       250        260        270        280        290        300 
DIDDTTDKMT TFTMNKFADL SVLEEDDDDE DEDEELEGEK EEEEEEKEKP EISNYDETEG 

       310        320        330        340        350        360 
KVNFSLEDSE SLEFDEGSDD YDYAKLTTEL VGNTDSLAED EDDILEEDED EDEEELLAIF 

       370        380        390        400        410        420 
DASDDSEIEA YLSIGDTLQN DFTIYDYDSE DEEYNNPSTQ KTEKVSQSKK GAKVKEEKGL 

       430        440        450        460        470        480 
KKDRKLPKKM RKAQKKLERK AGKAVAARMG DAFSNSLDDE SMNLYAELQE QWLKDKSKKA 

       490        500        510        520        530        540 
RRRAEREKLR SEGLLGKKSK KKLLRESQKP SSSDSDNASL TRIDKIFIND VYQRMQQFKH 

       550        560        570        580        590        600 
SAIEEISLPP CRKYVRRLVH ALANDLNLKS RSYGSGNKRY TMLSKTHKFD ASSVDLVSLT 

       610        620        630        640        650        660 
RIMERLQTRV EYQSFSKSGR KSRMTVSSVR SSKATRVYDG QIVGEDAPEI SKENPGRRLL 

       670        680        690 
EKLGWYAGKG LGHPENEGSK DSLRAIVKVS RSGLG 

References and documentation are available.

Number of amino acids: 695

Molecular weight: 79052.29

Theoretical pI: 4.80

Amino acid composition: 
Ala (A)  40	  5.8%
Arg (R)  36	  5.2%
Asn (N)  37	  5.3%
Asp (D)  65	  9.4%
Cys (C)   4	  0.6%
Gln (Q)  26	  3.7%
Glu (E)  83	 11.9%
Gly (G)  29	  4.2%
His (H)   7	  1.0%
Ile (I)  31	  4.5%
Leu (L)  62	  8.9%
Lys (K)  70	 10.1%
Met (M)  12	  1.7%
Phe (F)  20	  2.9%
Pro (P)  22	  3.2%
Ser (S)  72	 10.4%
Thr (T)  31	  4.5%
Trp (W)   3	  0.4%
Tyr (Y)  19	  2.7%
Val (V)  26	  3.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 148
Total number of positively charged residues (Arg + Lys): 106

Atomic composition:

Carbon      C	      3403
Hydrogen    H	      5443
Nitrogen    N	       953
Oxygen      O	      1177
Sulfur      S	        16

Formula: C3403H5443N953O1177S16
Total number of atoms: 10992

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45060
Abs 0.1% (=1 g/l)   0.570, assuming all pairs of Cys residues form cystines


Ext. coefficient    44810
Abs 0.1% (=1 g/l)   0.567, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.44
This classifies the protein as unstable.



Aliphatic index: 68.79

Grand average of hydropathicity (GRAVY): -1.013