ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNFRDSSSP RRGYSEFDPE SGEGLGRKKS LIRPERSRMD ESHPRFHYTQ VANQESNHIK 

        70         80         90        100        110        120 
VQPSSTGVDP RKSNELSTSR SHLSNYATPP HQEEEEDEGI PLMDIHNASP NVSSDQNNDL 

       130        140        150        160        170        180 
KGGREVYGLN DEINDYGSSP KKNQVISSSR PMNNEKPAKP KHDIYFWKVY CYAITFWAPA 

       190        200        210        220        230        240 
PLLKLFGLPT KDRQFAWREK IGLISCILYV GAFVAYLTFG FTKTVCSSQV VRTQINHVNG 

       250        260        270        280        290        300 
GYLIINGRAY DLTSSQHPKA AGIQAGSNVL YPPMNAGGKD ASFLFQNVNG NCKGLIKPRD 

       310        320        330        340        350        360 
NCSIPYDGDE LAWYMPCRLF NQDGSTKPNN TFAYYKGWAC HTSETARDAY YKLKVNGDVY 

       370        380        390        400        410        420 
FTWDDVKNSS RNLVVYSGNV LDLDLINWIE TDDVTYPELF DKLRDDETYR GLDISLVLTN 

       430        440        450        460        470        480 
SEERQAARCL TEIIKVGSID TDTIGCIASK VVLYMSLVFI LSVVVVKFIM ACWFKWVTSR 

       490        500        510        520        530        540 
KQGATMYDSK AWAKRNREIE DWVDHDHGIG AEVKTVPVKA RANYKAAKTN RQSVFHRAQK 

       550        560        570        580        590        600 
LSLGPNADLS QYYDNPNALS KTFKYTTMST QAALLGRNGY GKRGNNANKS VSGGFNGRQS 

       610        620        630        640        650        660 
NLYLTDQGSS TDLLNRPVSS YNPFDSMGDD SIVINGLSPD IIHPDVVPQP PVEYQPFGYP 

       670        680        690        700        710        720 
LAHTINLVTC YSEDEEGIRI TLDSIATTDY PNSHKLILVI CDGIIKGSGN DETTPDIVLD 

       730        740        750        760        770        780 
MMSDLTVPRD EVEAYSYVAV AQGSKRHNMA KVYAGFYKYN DETVPPEKQQ RIPMITIVKC 

       790        800        810        820        830        840 
GTPEEASAPK PGNRGKRDSQ IILMSFLQKV VFDERMTSLE YEMLQSIWRI TGLMAEFYEI 

       850        860        870        880        890        900 
VLMVDADTKV FPDSLTHMVA EMVKDPTIMG LCGETKISNK AQTWVTAIQV FEYYISHHQA 

       910        920        930        940        950        960 
KAFESIFGGV TCLPGCFCMY RIKAPKGSDG YWVPILANPD IVERYSDNVV DTLHRKNLLL 

       970        980        990       1000       1010       1020 
LGEDRYLSSL MLRTFPTRKQ VFVPKAACKT VVPDKFKVLL SQRRRWINST VHNLFELVLV 

      1030       1040       1050       1060       1070       1080 
KDLCGTFCFS MQFVIFIELI GTLVLPAAIT FTIYVIIVAI VSKPTPVMSL VLLAVIFGLP 

      1090       1100       1110       1120       1130       1140 
GCLIVITVSS LSYLVYFVIY LFALPIWNFV LPSYAYWKFD DFSWGETRTV AGGDKGDHSA 

      1150       1160       1170       1180       1190       1200 
VEGKFDSSKI AMKRWREWER ERRSTENRKQ QQQQQLTNNS SNNLAVPGAA WDPSNTGGNL 

      1210 
IDDLSQGSSS GSS 

References and documentation are available.

Number of amino acids: 1213

Molecular weight: 136207.78

Theoretical pI: 6.78

Amino acid composition: 
Ala (A)  71	  5.9%
Arg (R)  58	  4.8%
Asn (N)  67	  5.5%
Asp (D)  76	  6.3%
Cys (C)  21	  1.7%
Gln (Q)  42	  3.5%
Glu (E)  57	  4.7%
Gly (G)  78	  6.4%
His (H)  23	  1.9%
Ile (I)  74	  6.1%
Leu (L)  94	  7.7%
Lys (K)  72	  5.9%
Met (M)  28	  2.3%
Phe (F)  49	  4.0%
Pro (P)  63	  5.2%
Ser (S) 104	  8.6%
Thr (T)  69	  5.7%
Trp (W)  21	  1.7%
Tyr (Y)  55	  4.5%
Val (V)  91	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 133
Total number of positively charged residues (Arg + Lys): 130

Atomic composition:

Carbon      C	      6090
Hydrogen    H	      9443
Nitrogen    N	      1635
Oxygen      O	      1817
Sulfur      S	        49

Formula: C6090H9443N1635O1817S49
Total number of atoms: 19034

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   198700
Abs 0.1% (=1 g/l)   1.459, assuming all pairs of Cys residues form cystines


Ext. coefficient   197450
Abs 0.1% (=1 g/l)   1.450, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.83
This classifies the protein as unstable.



Aliphatic index: 81.62

Grand average of hydropathicity (GRAVY): -0.308