ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNGAFDAIF EYAWGQIDKP ISGDFIYGKD LPKLIEIIEN IFQKAQKSGS YELRLPLFSE 

        70         80         90        100        110        120 
INKDLFRTFS NTKTFFKIHK EEFDDIFFNL VNHPLREILE NAFIGVDSIP SDFIVSMNLN 

       130        140        150        160        170        180 
SPSKFLVENK NKNTEGAGIS TPRKKLTESP IKLLSRNNIG KALEVQVEEL KRELTAKQSL 

       190        200        210        220        230        240 
LQENERQVSE LKIRLETYQE KYASIQQRFS DLQKARQVED NQNSSRTSDP GSPLVTGIDQ 

       250        260        270        280        290        300 
KAILEEFRRR LQRQTDTISF LKDQIRRERG LNCSNDKVSH SKRKHATTDG DGTFKNFISA 

       310        320        330        340        350        360 
VPSNIWVKAT IRIIVCFALL AGVLPYIRKY VYAHDTPSQN SRLQLSWWEN SGILSKIVWF 

       370        380 
FEDQTDLETE YRSNANVDDA YSRVFGI 

References and documentation are available.

Number of amino acids: 387

Molecular weight: 44585.46

Theoretical pI: 8.28

Amino acid composition: 
Ala (A)  19	  4.9%
Arg (R)  24	  6.2%
Asn (N)  25	  6.5%
Asp (D)  23	  5.9%
Cys (C)   2	  0.5%
Gln (Q)  20	  5.2%
Glu (E)  29	  7.5%
Gly (G)  17	  4.4%
His (H)   5	  1.3%
Ile (I)  33	  8.5%
Leu (L)  34	  8.8%
Lys (K)  30	  7.8%
Met (M)   2	  0.5%
Phe (F)  24	  6.2%
Pro (P)  13	  3.4%
Ser (S)  35	  9.0%
Thr (T)  19	  4.9%
Trp (W)   5	  1.3%
Tyr (Y)  10	  2.6%
Val (V)  18	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      1997
Hydrogen    H	      3141
Nitrogen    N	       549
Oxygen      O	       601
Sulfur      S	         4

Formula: C1997H3141N549O601S4
Total number of atoms: 6292

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42525
Abs 0.1% (=1 g/l)   0.954, assuming all pairs of Cys residues form cystines


Ext. coefficient    42400
Abs 0.1% (=1 g/l)   0.951, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.73
This classifies the protein as unstable.



Aliphatic index: 85.92

Grand average of hydropathicity (GRAVY): -0.526