ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNHIQHLFI INRAGSLIYS WEARTETPEV EKTYEWPIDV HLEEVDQKAV VVFGEKDGIK 

        70         80         90        100        110        120 
LRYYVKSVNG ETVSGTRLQL GAQQVNVLEY LQEEDHYPVT VKFAAPTVST NEKIILSSMF 

       130        140        150        160        170        180 
HSLFTIAVQL SPCQKSSGVE VLETTQFKLF CLQSRTGVKF VVITSAASNI AADSLLSKMY 

       190        200        210        220 
ELYTDFALKN PFYSIDMPIR AQKFDEAIKT LLERAEKNNG AVTF 

References and documentation are available.

Number of amino acids: 224

Molecular weight: 25312.94

Theoretical pI: 5.60

Amino acid composition: 
Ala (A)  16	  7.1%
Arg (R)   7	  3.1%
Asn (N)   9	  4.0%
Asp (D)   8	  3.6%
Cys (C)   2	  0.9%
Gln (Q)  11	  4.9%
Glu (E)  19	  8.5%
Gly (G)   9	  4.0%
His (H)   5	  2.2%
Ile (I)  14	  6.2%
Leu (L)  20	  8.9%
Lys (K)  15	  6.7%
Met (M)   4	  1.8%
Phe (F)  12	  5.4%
Pro (P)   7	  3.1%
Ser (S)  18	  8.0%
Thr (T)  16	  7.1%
Trp (W)   2	  0.9%
Tyr (Y)   9	  4.0%
Val (V)  21	  9.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	      1145
Hydrogen    H	      1792
Nitrogen    N	       292
Oxygen      O	       342
Sulfur      S	         6

Formula: C1145H1792N292O342S6
Total number of atoms: 3577

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24535
Abs 0.1% (=1 g/l)   0.969, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.964, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.02
This classifies the protein as stable.



Aliphatic index: 93.53

Grand average of hydropathicity (GRAVY): -0.099