ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNKLFRLDA GYQQYDWGKI GSSSAVAQFA AHSDPSVQIE QDKPYAELWM GTHSKMPSYN 

        70         80         90        100        110        120 
HESKESLRDI ISKNPSAMLG KDIIDKFHAT NELPFLFKVL SIEKVLSIQA HPDKALGKIL 

       130        140        150        160        170        180 
HAQDPKNYPD DNHKPEMAIA VTDFEGFCGF KPLQEIADEL KRIPELRNIV GEETSRNFIE 

       190        200        210        220        230        240 
NIQPSAQKGS PEDEQNKKLL QAVFSRVMNA SDDKIKIQAR SLVERSKNSP SDFNKPDLPE 

       250        260        270        280        290        300 
LIQRLNKQFP DDVGLFCGCL LLNHCRLNAG EAIFLRAKDP HAYISGDIME CMAASDNVVR 

       310        320        330        340        350        360 
AGFTPKFKDV KNLVSMLTYT YDPVEKQKMQ PLKFDRSSGN GKSVLYNPPI EEFAVLETTF 

       370        380        390        400        410        420 
DEKLGQRHFE GVDGPSILIT TKGNGYIKAD GQKLKAEPGF VFFIAPHLPV DLEAEDEAFT 


TYRAFVEPN 

References and documentation are available.

Number of amino acids: 429

Molecular weight: 48188.73

Theoretical pI: 5.65

Amino acid composition: 
Ala (A)  32	  7.5%
Arg (R)  15	  3.5%
Asn (N)  22	  5.1%
Asp (D)  31	  7.2%
Cys (C)   5	  1.2%
Gln (Q)  19	  4.4%
Glu (E)  31	  7.2%
Gly (G)  24	  5.6%
His (H)  11	  2.6%
Ile (I)  26	  6.1%
Leu (L)  36	  8.4%
Lys (K)  37	  8.6%
Met (M)  10	  2.3%
Phe (F)  25	  5.8%
Pro (P)  28	  6.5%
Ser (S)  30	  7.0%
Thr (T)  13	  3.0%
Trp (W)   2	  0.5%
Tyr (Y)  11	  2.6%
Val (V)  21	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      2154
Hydrogen    H	      3358
Nitrogen    N	       576
Oxygen      O	       649
Sulfur      S	        15

Formula: C2154H3358N576O649S15
Total number of atoms: 6752

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27640
Abs 0.1% (=1 g/l)   0.574, assuming all pairs of Cys residues form cystines


Ext. coefficient    27390
Abs 0.1% (=1 g/l)   0.568, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.72
This classifies the protein as unstable.



Aliphatic index: 78.02

Grand average of hydropathicity (GRAVY): -0.490