ProtParam
User-provided sequence:
10 20 30 40 50 60
MSNKVKTKAM VPPINCIFNF LQQQTPVTIW LFEQIGIRIK GKIVGFDEFM NVVIDEAVEI
70 80 90
PVNSADGKED VEKGTPLGKI LLKGDNITLI TSAD
References and
documentation are available.
Number of amino acids: 94
Molecular weight: 10375.14
Theoretical pI: 4.99
Total number of negatively charged residues (Asp + Glu): 12
Total number of positively charged residues (Arg + Lys): 10
Atomic composition:
Carbon C 469
Hydrogen H 761
Nitrogen N 117
Oxygen O 138
Sulfur S 4
Formula: C
469H
761N
117O
138S
4
Total number of atoms: 1489
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 0.530, assuming all pairs of Cys residues form cystines
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 0.530, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 29.57
This classifies the protein as stable.
Aliphatic index: 106.70
Grand average of hydropathicity (GRAVY): 0.097