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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNKVKTKAM VPPINCIFNF LQQQTPVTIW LFEQIGIRIK GKIVGFDEFM NVVIDEAVEI

70 80 90
PVNSADGKED VEKGTPLGKI LLKGDNITLI TSAD


References and documentation are available.

Number of amino acids: 94 Molecular weight: 10375.14 Theoretical pI: 4.99
Amino acid composition: 
Ala (A) 4 4.3% Arg (R) 1 1.1% Asn (N) 6 6.4% Asp (D) 6 6.4% Cys (C) 1 1.1% Gln (Q) 4 4.3% Glu (E) 6 6.4% Gly (G) 7 7.4% His (H) 0 0.0% Ile (I) 12 12.8% Leu (L) 6 6.4% Lys (K) 9 9.6% Met (M) 3 3.2% Phe (F) 5 5.3% Pro (P) 5 5.3% Ser (S) 3 3.2% Thr (T) 6 6.4% Trp (W) 1 1.1% Tyr (Y) 0 0.0% Val (V) 9 9.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 12 Total number of positively charged residues (Arg + Lys): 10 Atomic composition: Carbon C 469 Hydrogen H 761 Nitrogen N 117 Oxygen O 138 Sulfur S 4 Formula: C469H761N117O138S4 Total number of atoms: 1489 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 5500 Abs 0.1% (=1 g/l) 0.530, assuming all pairs of Cys residues form cystines Ext. coefficient 5500 Abs 0.1% (=1 g/l) 0.530, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 29.57 This classifies the protein as stable. Aliphatic index: 106.70 Grand average of hydropathicity (GRAVY): 0.097