ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNMSQDDVT TGLRTVQQGL EALREEHSTI SNTLETSVKG VKEDEAPLPK QKLSQINDNL 

        70         80         90        100        110        120 
DKLVCGVDET SLMLMVFQLT QGMDAQHQKY QAQRRRLCQE NAWLRDELSS TQIKLQQSEQ 

       130        140        150        160        170        180 
MVAQLEEENK HLKYMASIKQ FDDGTQSDTK TSVDVGPQPV TNETLQELGF GPEDEEDMNA 

       190        200        210        220        230        240 
SQFNQPTPAN QMAASANVGY EIPARLRTLH NLVIQYASQG RYEVAVPLCK QALEDLEKTS 

       250        260        270        280        290        300 
GHDHPDVATM LNILALVYRD QNKYKEAANL LNEALSIREK CLGESHPAVA ATLNNLAVLF 

       310        320        330        340        350        360 
GKRGKFKDAE PLCKRALEIR EKVLGDDHPD VAKQLNNLAL LCQNQGKYEE VEKYYKRALE 

       370        380        390        400        410        420 
IYESKLGPDD PNVAKTKNNL SSAYLKQGKY KEAEELYKQI LTRAHEREFG QISGENKPIW 

       430        440        450        460        470        480 
QIAEEREENK HKGEGATANE QAGWAKAAKV DSPTVTTTLK NLGALYRRQG KYEAAETLED 

       490        500 
VALRAKKQMS ESRRMERSVM Y 

References and documentation are available.

Number of amino acids: 501

Molecular weight: 56464.50

Theoretical pI: 5.54

Amino acid composition: 
Ala (A)  46	  9.2%
Arg (R)  24	  4.8%
Asn (N)  28	  5.6%
Asp (D)  26	  5.2%
Cys (C)   6	  1.2%
Gln (Q)  38	  7.6%
Glu (E)  51	 10.2%
Gly (G)  26	  5.2%
His (H)  10	  2.0%
Ile (I)  14	  2.8%
Leu (L)  53	 10.6%
Lys (K)  41	  8.2%
Met (M)  13	  2.6%
Phe (F)   7	  1.4%
Pro (P)  17	  3.4%
Ser (S)  27	  5.4%
Thr (T)  27	  5.4%
Trp (W)   3	  0.6%
Tyr (Y)  17	  3.4%
Val (V)  27	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 65

Atomic composition:

Carbon      C	      2444
Hydrogen    H	      3935
Nitrogen    N	       703
Oxygen      O	       793
Sulfur      S	        19

Formula: C2444H3935N703O793S19
Total number of atoms: 7894

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42205
Abs 0.1% (=1 g/l)   0.747, assuming all pairs of Cys residues form cystines


Ext. coefficient    41830
Abs 0.1% (=1 g/l)   0.741, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.82
This classifies the protein as unstable.



Aliphatic index: 76.97

Grand average of hydropathicity (GRAVY): -0.765