ProtParam
User-provided sequence:
10 20 30 40 50 60
MSNNELLEIE PMYLQFPFEL KKQMSCSLYL TNKTDNNVAF KVKTTNRNNY CVRPNYGLIL
70 80 90 100 110 120
PKSTCKVLVT MQAQKEVPSD MQSFEKFMIQ SVLASPGVTA KEVTREMFSK ESGHVVEETK
130 140
LRVTYVCSTT TNITSSPRTR RGFIFQCFC
References and
documentation are available.
Number of amino acids: 149
Molecular weight: 17092.82
Theoretical pI: 9.07
Total number of negatively charged residues (Asp + Glu): 13
Total number of positively charged residues (Arg + Lys): 19
Atomic composition:
Carbon C 752
Hydrogen H 1203
Nitrogen N 201
Oxygen O 226
Sulfur S 13
Formula: C
752H
1203N
201O
226S
13
Total number of atoms: 2395
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7825
Abs 0.1% (=1 g/l) 0.458, assuming all pairs of Cys residues form cystines
Ext. coefficient 7450
Abs 0.1% (=1 g/l) 0.436, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.57
This classifies the protein as unstable.
Aliphatic index: 69.87
Grand average of hydropathicity (GRAVY): -0.316