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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNNIEDDSI SVDNIVDRLL EVRGSRPGKQ VTLTENEIRF LCTKAREIFI QQPILLELEA

70 80 90 100 110 120
PIKICGDIHG QYYDLLRLFE YGGFPPEANY LFLGDYVDRG KQSLETICLL LAYKIKYPEN

130 140 150 160 170 180
FFILRGNHEC ASINRIYGFY DECKRRFNIK LWKTFTDCFN CLPIAAIIDE KIFTMHGGLS

190 200 210 220 230 240
PDLNSMEQIR RVMRPTDIPD VGLLCDLLWS DPDKDITGWS ENDRGVSFTF GPDVVSRFLQ

250 260 270 280 290 300
KHDMDLICRA HQVVEDGYEF FSKRQLVTLF SAPNYCGEFD NAGAMMSVDE SLLCSFQILK

310 320 330
PADKKPRYAP ANVANNRPGA NQRKPKKAVK


References and documentation are available.

Number of amino acids: 330 Molecular weight: 37824.39 Theoretical pI: 5.68
Amino acid composition: 
Ala (A) 17 5.2% Arg (R) 21 6.4% Asn (N) 18 5.5% Asp (D) 26 7.9% Cys (C) 11 3.3% Gln (Q) 11 3.3% Glu (E) 20 6.1% Gly (G) 20 6.1% His (H) 5 1.5% Ile (I) 25 7.6% Leu (L) 32 9.7% Lys (K) 20 6.1% Met (M) 7 2.1% Phe (F) 20 6.1% Pro (P) 18 5.5% Ser (S) 17 5.2% Thr (T) 11 3.3% Trp (W) 3 0.9% Tyr (Y) 12 3.6% Val (V) 16 4.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 46 Total number of positively charged residues (Arg + Lys): 41 Atomic composition: Carbon C 1694 Hydrogen H 2635 Nitrogen N 455 Oxygen O 492 Sulfur S 18 Formula: C1694H2635N455O492S18 Total number of atoms: 5294 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 35005 Abs 0.1% (=1 g/l) 0.925, assuming all pairs of Cys residues form cystines Ext. coefficient 34380 Abs 0.1% (=1 g/l) 0.909, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 47.08 This classifies the protein as unstable. Aliphatic index: 86.58 Grand average of hydropathicity (GRAVY): -0.299