ProtParam
User-provided sequence:
10 20 30 40 50 60
MSNNIEDDSI SVDNIVDRLL EVRGSRPGKQ VTLTENEIRF LCTKAREIFI QQPILLELEA
70 80 90 100 110 120
PIKICGDIHG QYYDLLRLFE YGGFPPEANY LFLGDYVDRG KQSLETICLL LAYKIKYPEN
130 140 150 160 170 180
FFILRGNHEC ASINRIYGFY DECKRRFNIK LWKTFTDCFN CLPIAAIIDE KIFTMHGGLS
190 200 210 220 230 240
PDLNSMEQIR RVMRPTDIPD VGLLCDLLWS DPDKDITGWS ENDRGVSFTF GPDVVSRFLQ
250 260 270 280 290 300
KHDMDLICRA HQVVEDGYEF FSKRQLVTLF SAPNYCGEFD NAGAMMSVDE SLLCSFQILK
310 320 330
PADKKPRYAP ANVANNRPGA NQRKPKKAVK
References and
documentation are available.
Number of amino acids: 330
Molecular weight: 37824.39
Theoretical pI: 5.68
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1694
Hydrogen H 2635
Nitrogen N 455
Oxygen O 492
Sulfur S 18
Formula: C
1694H
2635N
455O
492S
18
Total number of atoms: 5294
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 35005
Abs 0.1% (=1 g/l) 0.925, assuming all pairs of Cys residues form cystines
Ext. coefficient 34380
Abs 0.1% (=1 g/l) 0.909, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.08
This classifies the protein as unstable.
Aliphatic index: 86.58
Grand average of hydropathicity (GRAVY): -0.299