ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNNKAGGEY EVITIGDAKK LQATISELGA TLLDLKVNNE SIVLGYPDIH GYISDGYNYI 

        70         80         90        100        110        120 
GATVGRYANR IYKGMFSMED GPHQLTVNNC GNTNHSSISS FHLKKYKASK VQNPLDDLYI 

       130        140        150        160        170        180 
VEFTLLDDRT LPNEFPGDLA VNLKYTLNVA DMTLDLEYEA KLVSGEATPI NMTNHTYFNL 

       190        200        210        220        230        240 
NKTMDKKSIS GTEVRLCSDK SLEVSEGALI PTGKIVQRKI ATFDSSKPTI LQDDGPIYDY 

       250        260        270        280        290        300 
AFIVDENKNL KTTDSVSVNK LVPAFKAYHP ASRLSLEVST TEPTVLFYTG DNLCDGFTPR 

       310        320        330        340 
SGFAVEQGRY VDAINRDGWR DCVLLRRGEV YTSKTRYRFA V 

References and documentation are available.

Number of amino acids: 341

Molecular weight: 37932.79

Theoretical pI: 5.61

Amino acid composition: 
Ala (A)  20	  5.9%
Arg (R)  14	  4.1%
Asn (N)  23	  6.7%
Asp (D)  25	  7.3%
Cys (C)   4	  1.2%
Gln (Q)   6	  1.8%
Glu (E)  18	  5.3%
Gly (G)  24	  7.0%
His (H)   6	  1.8%
Ile (I)  19	  5.6%
Leu (L)  32	  9.4%
Lys (K)  23	  6.7%
Met (M)   6	  1.8%
Phe (F)  12	  3.5%
Pro (P)  13	  3.8%
Ser (S)  25	  7.3%
Thr (T)  27	  7.9%
Trp (W)   1	  0.3%
Tyr (Y)  19	  5.6%
Val (V)  24	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1684
Hydrogen    H	      2642
Nitrogen    N	       448
Oxygen      O	       528
Sulfur      S	        10

Formula: C1684H2642N448O528S10
Total number of atoms: 5312

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34060
Abs 0.1% (=1 g/l)   0.898, assuming all pairs of Cys residues form cystines


Ext. coefficient    33810
Abs 0.1% (=1 g/l)   0.891, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 21.69
This classifies the protein as stable.



Aliphatic index: 84.60

Grand average of hydropathicity (GRAVY): -0.352