ProtParam
User-provided sequence:
10 20 30 40 50 60
MSNNNNSPTT VNQETTTSRE VSITLPTDQS PQTSPGSSSS PSPRPSGGSP ARRTATGLSG
70 80 90 100 110 120
KHSIFRGIRL RNGKWVSEIR EPRKTTRIWL GTYPVPEMAA AAYDVAALAL KGPDAVLNFP
130 140 150 160 170 180
GLALTYVAPV SNSAADIRAA ASRAAEMKQP DQGGDEKVLE PVQPGKEEEL EEVSCNSCSL
190 200 210 220 230
EFMDEEAMLN MPTLLTEMAE GMLMSPPRMM IHPTMEDDSP ENHEGDNLWS YK
References and
documentation are available.
Number of amino acids: 232
Molecular weight: 25106.10
Theoretical pI: 4.86
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 21
Atomic composition:
Carbon C 1074
Hydrogen H 1714
Nitrogen N 306
Oxygen O 359
Sulfur S 14
Formula: C
1074H
1714N
306O
359S
14
Total number of atoms: 3467
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22585
Abs 0.1% (=1 g/l) 0.900, assuming all pairs of Cys residues form cystines
Ext. coefficient 22460
Abs 0.1% (=1 g/l) 0.895, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 68.59
This classifies the protein as unstable.
Aliphatic index: 63.15
Grand average of hydropathicity (GRAVY): -0.584