ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNPDDLDDG LAYDFDAEHE VIFDAKDGSP PTKKVQKRSI EQDDDDVDDI DGKKEERNSE 

        70         80         90        100        110        120 
DDSNRPISKR QKKLQKKSKL IEKKKEESQY IVSQRKALPA SSPEKIIEYL TTLIREKNPD 

       130        140        150        160        170        180 
LSVLELEELY FKRNDFLSTE KFDAERRLSN FPAFIQKFSV APKKIVFSMS NIRVADVYRS 

       190        200        210        220        230        240 
LNGGKNCVKL FSKSKLKDDI ATVERLLTDS SKKSNKNKDS LYFIATPTRM QKIIEATDLL 

       250        260        270        280        290 
FQGKEKLDII LDASYLDPKD NTILSFENAA VLCQVLKTFL NKKSSVKILL Y 

References and documentation are available.

Number of amino acids: 291

Molecular weight: 33394.09

Theoretical pI: 8.22

Amino acid composition: 
Ala (A)  15	  5.2%
Arg (R)  13	  4.5%
Asn (N)  14	  4.8%
Asp (D)  30	 10.3%
Cys (C)   2	  0.7%
Gln (Q)  10	  3.4%
Glu (E)  21	  7.2%
Gly (G)   6	  2.1%
His (H)   1	  0.3%
Ile (I)  20	  6.9%
Leu (L)  30	 10.3%
Lys (K)  40	 13.7%
Met (M)   3	  1.0%
Phe (F)  14	  4.8%
Pro (P)  11	  3.8%
Ser (S)  28	  9.6%
Thr (T)  11	  3.8%
Trp (W)   0	  0.0%
Tyr (Y)   8	  2.7%
Val (V)  14	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      1484
Hydrogen    H	      2404
Nitrogen    N	       396
Oxygen      O	       465
Sulfur      S	         5

Formula: C1484H2404N396O465S5
Total number of atoms: 4754

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12045
Abs 0.1% (=1 g/l)   0.361, assuming all pairs of Cys residues form cystines


Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.357, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.22
This classifies the protein as unstable.



Aliphatic index: 86.12

Grand average of hydropathicity (GRAVY): -0.691