ProtParam
User-provided sequence:
10 20 30 40 50 60
MSNPFQNIGK NLLYISAAGI ASIYVVKTIV KARRDAKFIP KARGNNGEVN EKNYYDNLAQ
70 80 90 100 110
VKPGFPIPKD GGDNIDCSED HQLVRKSKYE GSGLSAVTRK RGDKLGFLDR RRNE
References and
documentation are available.
Number of amino acids: 114
Molecular weight: 12714.43
Theoretical pI: 9.75
Total number of negatively charged residues (Asp + Glu): 13
Total number of positively charged residues (Arg + Lys): 21
Atomic composition:
Carbon C 558
Hydrogen H 900
Nitrogen N 168
Oxygen O 168
Sulfur S 2
Formula: C
558H
900N
168O
168S
2
Total number of atoms: 1796
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7450
Abs 0.1% (=1 g/l) 0.586, assuming all pairs of Cys residues form cystines
Ext. coefficient 7450
Abs 0.1% (=1 g/l) 0.586, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.65
This classifies the protein as stable.
Aliphatic index: 76.14
Grand average of hydropathicity (GRAVY): -0.749