ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNPIENTEN SQNTSSSRFL RNVQRLALNN VTNTTFQKSN ANNPALTNFK STLNSVKKEG 

        70         80         90        100        110        120 
SRIPQFTRES VSRSTAAQEE KRTLKENGIQ LPKNNLLDDK ENQDPSSQQF GALTSIKEGR 

       130        140        150        160        170        180 
AELPANISLQ ESSSAKEIIQ HDPLKGVGSS TEVVHNSVEN EKLHPARSQL QVRNTESETD 

       190        200        210        220        230        240 
SGKKRPISTI VEQELPKKFK VCDENGKEEY EWEDLDAEDV NDPFMVSEYV NDIFEYLHQL 

       250        260        270        280        290        300 
EVITLPKKED LYQHRNIHQN RDILVNWLVK IHNKFGLLPE TLYLAINIMD RFLGKELVQL 

       310        320        330        340        350        360 
DKLQLVGTSC LFIASKYEEV YSPSIKHFAS ETDGACTEDE IKEGEKFILK TLKFNLNYPN 

       370        380        390        400        410        420 
PMNFLRRISK ADDYDIQSRT LAKFLLEISL VDFRFIGILP SLCAAAAMFM SRKMLGKGKW 

       430        440        450        460        470        480 
DGNLIHYSGG YTKEELAPVC HMIMDYLVSP IVHDEFHRKY QSRRFMKASI ISVQWALKVR 

       490 
KNGYDIMTLH E 

References and documentation are available.

Number of amino acids: 491

Molecular weight: 56246.81

Theoretical pI: 6.27

Amino acid composition: 
Ala (A)  24	  4.9%
Arg (R)  23	  4.7%
Asn (N)  35	  7.1%
Asp (D)  25	  5.1%
Cys (C)   5	  1.0%
Gln (Q)  22	  4.5%
Glu (E)  43	  8.8%
Gly (G)  20	  4.1%
His (H)  13	  2.6%
Ile (I)  31	  6.3%
Leu (L)  49	 10.0%
Lys (K)  40	  8.1%
Met (M)  11	  2.2%
Phe (F)  21	  4.3%
Pro (P)  19	  3.9%
Ser (S)  42	  8.6%
Thr (T)  24	  4.9%
Trp (W)   4	  0.8%
Tyr (Y)  14	  2.9%
Val (V)  26	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2489
Hydrogen    H	      3945
Nitrogen    N	       687
Oxygen      O	       765
Sulfur      S	        16

Formula: C2489H3945N687O765S16
Total number of atoms: 7902

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43110
Abs 0.1% (=1 g/l)   0.766, assuming all pairs of Cys residues form cystines


Ext. coefficient    42860
Abs 0.1% (=1 g/l)   0.762, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.12
This classifies the protein as unstable.



Aliphatic index: 83.79

Grand average of hydropathicity (GRAVY): -0.568