ProtParam
User-provided sequence:
10 20 30 40 50 60
MSNPYQNSQN GYQQNNSYEL NNYPNKQYSS SNEDDFVQFM NEIQDINSQL DNYSNIINLI
70 80 90 100 110 120
DNKQKNFLHG LDLNDEDTDY DSKQIENLVN EAQSLQLDLK NRIKNVQTQA VHSRDQTKVD
130 140 150 160 170 180
QAETCRKRFL DLIQDYRLVE ARNKESTKEQ AARQYQIIKP DATDEEIKAV VEDGSQQYFQ
190 200 210 220 230 240
QALMQSNRRG EARSVLNEVQ VRHRELLKLE KTMAELTQLF HDMEELVIEQ DQPIQQIEEQ
250 260 270 280 290
VGTAQHDIEQ GVGHTNKAVK SAKSARKKKL WCFFICLLIV IILAVILGAY FGTRK
References and
documentation are available.
Number of amino acids: 295
Molecular weight: 34308.28
Theoretical pI: 5.21
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 1490
Hydrogen H 2370
Nitrogen N 430
Oxygen O 484
Sulfur S 8
Formula: C
1490H
2370N
430O
484S
8
Total number of atoms: 4782
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22015
Abs 0.1% (=1 g/l) 0.642, assuming all pairs of Cys residues form cystines
Ext. coefficient 21890
Abs 0.1% (=1 g/l) 0.638, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.80
This classifies the protein as unstable.
Aliphatic index: 83.29
Grand average of hydropathicity (GRAVY): -0.819