ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNRKFGMEG FGINRQTSYS FERSQAPQRL YVPPSSRGGD NSEDADLDNI DYMENEEAEE 

        70         80         90        100        110        120 
DIEEGGSAAA SGGEVDEIDP LDAFMEGIHQ EMKSAPPPKP KEKLERYKDD DDDPVESYLK 

       130        140        150        160        170        180 
AKKDLGLTLA ADALNAGYNS DEEVYAAAKA VDAGMLDYDS DDNPIVVDKR KIEPITALDH 

       190        200        210        220        230        240 
SSIDYEPINK DFYEELESIS GMTEQETTDY RQRLGIRVSG FDVHRPVKTF EDCGFSSQIM 

       250        260        270        280        290        300 
SAIKKQAYEK PTAIQCQALP IVLSGRDVIG IAKTGSGKTA AFVLPMIVHI MDQPELQRDE 

       310        320        330        340        350        360 
GPIGVICAPT RELAHQIFLE AKKFSKAYGL RVSAVYGGMS KHEQFKELKA GCEIVVATPG 

       370        380        390        400        410        420 
RLIDMLKMKA LTMMRASYLV LDEADRMFDL GFEPQVRSIV GQIRPDRQTL LFSATMPWKV 

       430        440        450        460        470        480 
EKLAREILSD PIRVTVGEVG MANEDITQVV NVIPSDAEKL PWLLEKLPGM IDEGDVLVFA 

       490        500        510        520        530        540 
SKKATVDEIE AQLTLNSFKV AALHGDKDQA SRMETLQKFK SGVHHVLIAT DVAARGLDIK 

       550        560        570        580        590        600 
SLKTVVNYDI AKDMDMHVHR IGRTGRAGDR DGVAYTLVTQ REARFAGELV NSLVAAGQNV 

       610        620        630        640        650        660 
PPELTDLAMK DGRFKSKRDG RKGGKKGRGG GGGNKGVRGV DFGLGIGFSS ESSRTPSSKA 

       670        680        690        700        710        720 
APSRSGAINS VRTGVMAQFK NSFVAATPSN PQNQAYPNKR PSLMGFVSGG TIGGDMGRTQ 

       730        740        750        760 
SQAPPVAPTQ NASSHNSSQN HSQSSENRPR ERKRRSGWDN 

References and documentation are available.

Number of amino acids: 760

Molecular weight: 83050.62

Theoretical pI: 5.61

Amino acid composition: 
Ala (A)  67	  8.8%
Arg (R)  46	  6.1%
Asn (N)  26	  3.4%
Asp (D)  58	  7.6%
Cys (C)   4	  0.5%
Gln (Q)  31	  4.1%
Glu (E)  53	  7.0%
Gly (G)  67	  8.8%
His (H)  13	  1.7%
Ile (I)  40	  5.3%
Leu (L)  50	  6.6%
Lys (K)  51	  6.7%
Met (M)  26	  3.4%
Phe (F)  25	  3.3%
Pro (P)  39	  5.1%
Ser (S)  60	  7.9%
Thr (T)  32	  4.2%
Trp (W)   3	  0.4%
Tyr (Y)  18	  2.4%
Val (V)  51	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 111
Total number of positively charged residues (Arg + Lys): 97

Atomic composition:

Carbon      C	      3611
Hydrogen    H	      5775
Nitrogen    N	      1035
Oxygen      O	      1150
Sulfur      S	        30

Formula: C3611H5775N1035O1150S30
Total number of atoms: 11601

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43570
Abs 0.1% (=1 g/l)   0.525, assuming all pairs of Cys residues form cystines


Ext. coefficient    43320
Abs 0.1% (=1 g/l)   0.522, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.16
This classifies the protein as unstable.



Aliphatic index: 74.46

Grand average of hydropathicity (GRAVY): -0.509