ProtParam
User-provided sequence:
10 20 30 40 50 60
MSNTIVIVYL GANRIEIGRS ADACPQEIIA WKTGSINEKN REELKKIFEH YFQICNILGN
70 80 90 100 110 120
REVQVLILED IFISVVEKRI ICSILFKEFD CAHVSFVPRA IVHCLSCNTR NAIVIDIGTN
130 140 150 160 170 180
YTTCVPIFDL RPLQQFIKYS KRGKRQVESR IPLPGSLYMP IFFDEEYNSK NCEVDETPVI
190 200 210 220 230 240
NLVKNIVESL PIDLRRPLRE NIIIVNIEEA YETVIRNLFK LKMDTSKIQF PKNYWQAGSA
250 260 270 280
CAKILLHYKG SNIVGIERDE FYNNPHIAPD WFDYYFRTGV KRLQ
References and
documentation are available.
Number of amino acids: 284
Molecular weight: 33018.31
Theoretical pI: 8.01
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1497
Hydrogen H 2356
Nitrogen N 398
Oxygen O 419
Sulfur S 12
Formula: C
1497H
2356N
398O
419S
12
Total number of atoms: 4682
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34880
Abs 0.1% (=1 g/l) 1.056, assuming all pairs of Cys residues form cystines
Ext. coefficient 34380
Abs 0.1% (=1 g/l) 1.041, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 59.25
This classifies the protein as unstable.
Aliphatic index: 102.92
Grand average of hydropathicity (GRAVY): -0.112