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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNTIVIVYL GANRIEIGRS ADACPQEIIA WKTGSINEKN REELKKIFEH YFQICNILGN

70 80 90 100 110 120
REVQVLILED IFISVVEKRI ICSILFKEFD CAHVSFVPRA IVHCLSCNTR NAIVIDIGTN

130 140 150 160 170 180
YTTCVPIFDL RPLQQFIKYS KRGKRQVESR IPLPGSLYMP IFFDEEYNSK NCEVDETPVI

190 200 210 220 230 240
NLVKNIVESL PIDLRRPLRE NIIIVNIEEA YETVIRNLFK LKMDTSKIQF PKNYWQAGSA

250 260 270 280
CAKILLHYKG SNIVGIERDE FYNNPHIAPD WFDYYFRTGV KRLQ


References and documentation are available.

Number of amino acids: 284 Molecular weight: 33018.31 Theoretical pI: 8.01
Amino acid composition: 
Ala (A) 12 4.2% Arg (R) 18 6.3% Asn (N) 20 7.0% Asp (D) 12 4.2% Cys (C) 9 3.2% Gln (Q) 9 3.2% Glu (E) 22 7.7% Gly (G) 11 3.9% His (H) 5 1.8% Ile (I) 37 13.0% Leu (L) 20 7.0% Lys (K) 18 6.3% Met (M) 3 1.1% Phe (F) 15 5.3% Pro (P) 13 4.6% Ser (S) 15 5.3% Thr (T) 10 3.5% Trp (W) 3 1.1% Tyr (Y) 12 4.2% Val (V) 20 7.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 34 Total number of positively charged residues (Arg + Lys): 36 Atomic composition: Carbon C 1497 Hydrogen H 2356 Nitrogen N 398 Oxygen O 419 Sulfur S 12 Formula: C1497H2356N398O419S12 Total number of atoms: 4682 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 34880 Abs 0.1% (=1 g/l) 1.056, assuming all pairs of Cys residues form cystines Ext. coefficient 34380 Abs 0.1% (=1 g/l) 1.041, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 59.25 This classifies the protein as unstable. Aliphatic index: 102.92 Grand average of hydropathicity (GRAVY): -0.112