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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNTWDDVWA SDSDVETERS PDLVKLRENH SKRGYLDGIV SSKEEKLQEG FNDGFPTGAK

70 80 90 100 110 120
LGKQVGIIMG ILLGLRTRFG DEDEDLSKAY IDAQKELRIN KVLSKSIFDP NFDLQEKHPL

130 140
ITKWTDIANT YCEKYHVPSI Q


References and documentation are available.

Number of amino acids: 141 Molecular weight: 16076.05 Theoretical pI: 5.08
Amino acid composition: 
Ala (A) 5 3.5% Arg (R) 6 4.3% Asn (N) 6 4.3% Asp (D) 14 9.9% Cys (C) 1 0.7% Gln (Q) 5 3.5% Glu (E) 11 7.8% Gly (G) 10 7.1% His (H) 3 2.1% Ile (I) 10 7.1% Leu (L) 13 9.2% Lys (K) 13 9.2% Met (M) 2 1.4% Phe (F) 5 3.5% Pro (P) 5 3.5% Ser (S) 11 7.8% Thr (T) 7 5.0% Trp (W) 3 2.1% Tyr (Y) 4 2.8% Val (V) 7 5.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 25 Total number of positively charged residues (Arg + Lys): 19 Atomic composition: Carbon C 713 Hydrogen H 1118 Nitrogen N 192 Oxygen O 225 Sulfur S 3 Formula: C713H1118N192O225S3 Total number of atoms: 2251 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 22460 Abs 0.1% (=1 g/l) 1.397, assuming all pairs of Cys residues form cystines Ext. coefficient 22460 Abs 0.1% (=1 g/l) 1.397, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 38.26 This classifies the protein as stable. Aliphatic index: 81.56 Grand average of hydropathicity (GRAVY): -0.665