ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNVAGELKN SEGKKKGRGN RYHNKNRGKS KNETVDPKKN ENKVNNATNA THNNSKGRRN 

        70         80         90        100        110        120 
NKKRNREYYN YKRKARLGKS TENEGFKLVI RLLPPNLTAD EFFAILRDNN NDDGDKQDIQ 

       130        140        150        160        170        180 
GKLKYSDWCF FEGHYSSKVF KNSTYSRCNF LFDNLSDLEK CANFIKTCKF IDNKDNITIP 

       190        200        210        220        230        240 
DMKLSPYVKK FTQTSKKDAA LVGTIEEDEI FKTFMNSMKQ LNENDEYSFQ DFSVLKSLEK 

       250        260        270        280        290        300 
EFSKSIELEN KIAERTERVL TELVGTGDKV KNKNKKKKNK NAKKKFKEEE ASAKIPKKKR 

       310        320        330        340        350        360 
NRGKKKRENR EKSTISKTKN SNVVIIEEAG KEVLKQRKKK MLLQEKLKIS NSSQPQSSSA 

       370        380 
QTQPSFQPKE NLFVPRVKIL HRDDTKK 

References and documentation are available.

Number of amino acids: 387

Molecular weight: 44924.11

Theoretical pI: 9.89

Amino acid composition: 
Ala (A)  15	  3.9%
Arg (R)  21	  5.4%
Asn (N)  42	 10.9%
Asp (D)  20	  5.2%
Cys (C)   4	  1.0%
Gln (Q)  12	  3.1%
Glu (E)  32	  8.3%
Gly (G)  16	  4.1%
His (H)   4	  1.0%
Ile (I)  17	  4.4%
Leu (L)  25	  6.5%
Lys (K)  70	 18.1%
Met (M)   5	  1.3%
Phe (F)  19	  4.9%
Pro (P)  10	  2.6%
Ser (S)  30	  7.8%
Thr (T)  19	  4.9%
Trp (W)   1	  0.3%
Tyr (Y)   9	  2.3%
Val (V)  16	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 91

Atomic composition:

Carbon      C	      1963
Hydrogen    H	      3203
Nitrogen    N	       583
Oxygen      O	       604
Sulfur      S	         9

Formula: C1963H3203N583O604S9
Total number of atoms: 6362

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19160
Abs 0.1% (=1 g/l)   0.426, assuming all pairs of Cys residues form cystines


Ext. coefficient    18910
Abs 0.1% (=1 g/l)   0.421, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.74
This classifies the protein as stable.



Aliphatic index: 58.19

Grand average of hydropathicity (GRAVY): -1.254