ProtParam
User-provided sequence:
10 20 30 40 50 60
MSNVIGDRTE DGLSTAAAAS GSTAVQSSPP TDRPVRVYAD GIYDLFHFGH ARSLEQAKLA
70 80 90 100 110 120
FPNNTYLLVG CCNDETTHKY KGRTVMTAEE RYESLRHCKW VDEVIPDAPW VVNQEFLDKH
130 140 150 160 170 180
QIDYVAHDSL PYADSSGAGK DVYEFVKKVG RFKETQRTEG ISTSDIIMRI VKDYNQYVMR
190 200 210 220 230 240
NLDRGYSRED LGVSFVKEKR LRVNMRLKKL QERVKEQQER VGEKIQTVKM LRNEWVENAD
250 260 270 280 290 300
RWVAGFLEIF EEGCHKMGTA IVDSIQERLM RQKSAERLEN GQDDDTDDQF YEEYFDHDMG
310 320 330
SDDDEDEKFY DEEEVKEEET EKTVMTDAKD NK
References and
documentation are available.
Number of amino acids: 332
Molecular weight: 38485.67
Theoretical pI: 4.86
Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 47
Atomic composition:
Carbon C 1671
Hydrogen H 2597
Nitrogen N 471
Oxygen O 547
Sulfur S 14
Formula: C
1671H
2597N
471O
547S
14
Total number of atoms: 5300
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 43110
Abs 0.1% (=1 g/l) 1.120, assuming all pairs of Cys residues form cystines
Ext. coefficient 42860
Abs 0.1% (=1 g/l) 1.114, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.16
This classifies the protein as unstable.
Aliphatic index: 64.55
Grand average of hydropathicity (GRAVY): -0.874