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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNVIGDRTE DGLSTAAAAS GSTAVQSSPP TDRPVRVYAD GIYDLFHFGH ARSLEQAKLA

70 80 90 100 110 120
FPNNTYLLVG CCNDETTHKY KGRTVMTAEE RYESLRHCKW VDEVIPDAPW VVNQEFLDKH

130 140 150 160 170 180
QIDYVAHDSL PYADSSGAGK DVYEFVKKVG RFKETQRTEG ISTSDIIMRI VKDYNQYVMR

190 200 210 220 230 240
NLDRGYSRED LGVSFVKEKR LRVNMRLKKL QERVKEQQER VGEKIQTVKM LRNEWVENAD

250 260 270 280 290 300
RWVAGFLEIF EEGCHKMGTA IVDSIQERLM RQKSAERLEN GQDDDTDDQF YEEYFDHDMG

310 320 330
SDDDEDEKFY DEEEVKEEET EKTVMTDAKD NK


References and documentation are available.

Number of amino acids: 332 Molecular weight: 38485.67 Theoretical pI: 4.86
Amino acid composition: 
Ala (A) 19 5.7% Arg (R) 23 6.9% Asn (N) 12 3.6% Asp (D) 33 9.9% Cys (C) 4 1.2% Gln (Q) 14 4.2% Glu (E) 36 10.8% Gly (G) 19 5.7% His (H) 8 2.4% Ile (I) 12 3.6% Leu (L) 18 5.4% Lys (K) 24 7.2% Met (M) 10 3.0% Phe (F) 12 3.6% Pro (P) 7 2.1% Ser (S) 18 5.4% Thr (T) 18 5.4% Trp (W) 4 1.2% Tyr (Y) 14 4.2% Val (V) 27 8.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 69 Total number of positively charged residues (Arg + Lys): 47 Atomic composition: Carbon C 1671 Hydrogen H 2597 Nitrogen N 471 Oxygen O 547 Sulfur S 14 Formula: C1671H2597N471O547S14 Total number of atoms: 5300 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 43110 Abs 0.1% (=1 g/l) 1.120, assuming all pairs of Cys residues form cystines Ext. coefficient 42860 Abs 0.1% (=1 g/l) 1.114, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 40.16 This classifies the protein as unstable. Aliphatic index: 64.55 Grand average of hydropathicity (GRAVY): -0.874