ProtParam
User-provided sequence:
10 20 30 40 50 60
MSPFGSKKNR SLSVRVSTFD SELEFKLEPR ASGQDLFDLV CRTIGLRESW YFGLQYVDTR
70 80 90 100 110 120
SNVSWLKMEK RVRDQRVELH ASNNVYVFSF YAKFFPENVS EELIQEITQH LFFLQVKQSI
130 140 150 160 170 180
LSMDIYCRPE ASVLLASYAV HVQYGPYDYE TYKDGMLAGG ELLPKGVTDQ YQMTPEMWEE
190 200 210 220 230 240
RIKTWYMDHE PMTRDEVEME YLKIAQDLDM YGVNYFPITN KNKTKLWLGV TSVGLNIYDE
250 260 270 280 290 300
RDKLTPKTTF QWNEIRHVSF DDKKFTIRLV DAKVSNFIFY SQDLHINKMI LDLCKGNHDL
310 320 330 340 350 360
YMRRRKPDTM EIQQMKAQAK EEKQRRQIER KKFIREKKLR EKAEHERYEL EKSMEHLQNE
370 380 390 400 410 420
MRMANDALRR SEETKELYFE KSRVNEEQMQ LTECKANHFK TEMDRLRERQ MKIEREKHDL
430 440 450 460 470 480
EKKIRDADFY VHQLTVENDK REAETEKLRK ELICAKMAER EATARLLEFL NSGRKSSTDS
490 500 510 520 530 540
LLTASSVSHA ANTASSMAAI STPSLITSSS TNDLETAGGA ELTTHSSHYL VQGDNSSGIS
550 560 570 580 590 600
DDFEPKEFIL TDNEMEQITN EMERNHLDYL RNSKQVQSQL QTLRSEIAPH KIEENQSNLD
610 620 630
ILSEAQIKAG ENKYSTLKKL KSGSTKARVA FFEEL
References and
documentation are available.
Number of amino acids: 635
Molecular weight: 74492.25
Theoretical pI: 6.03
Total number of negatively charged residues (Asp + Glu): 105
Total number of positively charged residues (Arg + Lys): 95
Atomic composition:
Carbon C 3269
Hydrogen H 5168
Nitrogen N 914
Oxygen O 1018
Sulfur S 29
Formula: C
3269H
5168N
914O
1018S
29
Total number of atoms: 10398
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 69010
Abs 0.1% (=1 g/l) 0.926, assuming all pairs of Cys residues form cystines
Ext. coefficient 68760
Abs 0.1% (=1 g/l) 0.923, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.59
This classifies the protein as unstable.
Aliphatic index: 72.65
Grand average of hydropathicity (GRAVY): -0.776