ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSPKSLLITG ANRGIGLGLL KQFLKHKDIQ IIIATCRDPS KAEELSNLKD SRLHILPLDI 

        70         80         90        100        110        120 
DCDESISKLY AEVEKLVGED GLTVLLNNAG ILLPYDVEGE KNRKTLIRQL ETNSVSTALI 

       130        140        150        160        170        180 
TQEFLPLLKK AAAKNGGDGY SINRAAIVNI SSTAASVEKI DGTFNGPLVA YRMSKSALNS 

       190        200        210        220        230        240 
FAKSCSIDLA KYHILVTSFC PGWVKTGMGG ANAMLEIEDA TKTLSDNILT LGNAHHGAYL 


NADLTVIPN 

References and documentation are available.

Number of amino acids: 249

Molecular weight: 26766.84

Theoretical pI: 6.52

Amino acid composition: 
Ala (A)  23	  9.2%
Arg (R)   7	  2.8%
Asn (N)  16	  6.4%
Asp (D)  14	  5.6%
Cys (C)   4	  1.6%
Gln (Q)   4	  1.6%
Glu (E)  13	  5.2%
Gly (G)  19	  7.6%
His (H)   5	  2.0%
Ile (I)  21	  8.4%
Leu (L)  34	 13.7%
Lys (K)  19	  7.6%
Met (M)   4	  1.6%
Phe (F)   5	  2.0%
Pro (P)   8	  3.2%
Ser (S)  20	  8.0%
Thr (T)  15	  6.0%
Trp (W)   1	  0.4%
Tyr (Y)   6	  2.4%
Val (V)  11	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1185
Hydrogen    H	      1940
Nitrogen    N	       320
Oxygen      O	       365
Sulfur      S	         8

Formula: C1185H1940N320O365S8
Total number of atoms: 3818

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14690
Abs 0.1% (=1 g/l)   0.549, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.539, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.14
This classifies the protein as stable.



Aliphatic index: 108.19

Grand average of hydropathicity (GRAVY): 0.005