ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSPLTRTVAI KKTVKVLSKC QSGREYTQKF LQRAYSTSHA NSTYYSRTKL FISSHSKALN 

        70         80         90        100        110        120 
IALLSGSLLL TYSYYSPKKI LSLDTINGIK DYSTNTSGNI NMPSPNPKGT ETQKSQRSQN 

       130        140        150        160        170        180 
DQSVLILNDS KIEAKLHDRE ESHFVNRGTG IFRYDVAQLP SNHPIEDDHV EQIITIPIES 

       190        200        210        220        230        240 
EDGKSIEKDL YFFGIFDGHG GPFTSEKLSK DLVRYVAYQL GQVYDQNKTV FHSDPNQLID 

       250        260        270        280        290        300 
SAISKGFLKL DNDLVIESFR KLFQDPNNTN IANTLPAISG SCALLSLYNS TNSILKVAVT 

       310        320        330        340        350        360 
GDSRALICGL DNEGNWTVKS LSTDQTGDNL DEVRRIRKEH PGEPNVIRNG RILGSLQPSR 

       370        380        390        400        410        420 
AFGDYRYKIK EVDGKPLSDL PEVAKLYFRR EPRDFKTPPY VTAEPVITSA KIGENTKFMV 

       430        440        450        460        470        480 
MGSDGLFELL TNEEIASLVI RWMDKNMNLA PVKAEPGKLP KVIDVSEDKE AQRPAFRYKD 

       490        500        510        520        530        540 
NNSSSPSGSN PEYLIEDKNV ATHLIRNALS AGGRKEYVSA LVSIPSPMSR RYRDDLTVTV 

       550        560        570 
AFFGDSGTPS IVSNATSIVM NPEATTKPKP RL 

References and documentation are available.

Number of amino acids: 572

Molecular weight: 63668.92

Theoretical pI: 8.70

Amino acid composition: 
Ala (A)  30	  5.2%
Arg (R)  30	  5.2%
Asn (N)  36	  6.3%
Asp (D)  36	  6.3%
Cys (C)   3	  0.5%
Gln (Q)  17	  3.0%
Glu (E)  31	  5.4%
Gly (G)  33	  5.8%
His (H)  10	  1.7%
Ile (I)  38	  6.6%
Leu (L)  51	  8.9%
Lys (K)  42	  7.3%
Met (M)   8	  1.4%
Phe (F)  20	  3.5%
Pro (P)  33	  5.8%
Ser (S)  61	 10.7%
Thr (T)  36	  6.3%
Trp (W)   2	  0.3%
Tyr (Y)  22	  3.8%
Val (V)  33	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      2816
Hydrogen    H	      4483
Nitrogen    N	       779
Oxygen      O	       879
Sulfur      S	        11

Formula: C2816H4483N779O879S11
Total number of atoms: 8968

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43905
Abs 0.1% (=1 g/l)   0.690, assuming all pairs of Cys residues form cystines


Ext. coefficient    43780
Abs 0.1% (=1 g/l)   0.688, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.02
This classifies the protein as unstable.



Aliphatic index: 82.66

Grand average of hydropathicity (GRAVY): -0.498