ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSPRQLIPTL IPEWAPLSQQ SCIREDELDS PPITPTSQTS SFGSSFSQQK PTYSTIIGEN 

        70         80         90        100        110        120 
IHTILDEIRP YVKKITVSDQ DKKTINQYTL GVSAGSGQFG YVRKAYSSTL GKVVAVKIIP 

       130        140        150        160        170        180 
KKPWNAQQYS VNQVMRQIQL WKSKGKITTN MSGNEAMRLM NIEKCRWEIF AASRLRNNVH 

       190        200        210        220        230        240 
IVRLIECLDS PFSESIWIVT NWCSLGELQW KRDDDEDILP QWKKIVISNC SVSTFAKKIL 

       250        260        270        280        290        300 
EDMTKGLEYL HSQGCIHRDI KPSNILLDEE EKVAKLSDFG SCIFTPQSLP FSDANFEDCF 

       310        320        330        340        350        360 
QRELNKIVGT PAFIAPELCH LGNSKRDFVT DGFKLDIWSL GVTLYCLLYN ELPFFGENEF 

       370        380        390        400        410        420 
ETYHKIIEVS LSSKINGNTL NDLVIKRLLE KDVTLRISIQ DLVKVLSRDQ PIDSRNHSQI 

       430        440        450        460        470        480 
SSSSVNPVRN EGPVRRFFGR LLTKKGKKKT SGKGKDKVLV SATSKVTPSI HIDEEPDKEC 

       490        500        510        520        530        540 
FSTTVLRSSP DSSDYCSSLG EEAIQVTDFL DTFCRSNESL PNLTVNNDKQ NSDMKTDRSE 

       550        560        570        580        590        600 
SSSHSSLKIP TPIKAMIRLK SSPKENGNRT HINCSQDKPS SPLMDRTVGK RTVNNSGARK 

       610        620        630        640 
LAHSSNILNF KAYINSEDSD IRETVEDVKT YLNFADNGQI 

References and documentation are available.

Number of amino acids: 640

Molecular weight: 72149.92

Theoretical pI: 8.36

Amino acid composition: 
Ala (A)  20	  3.1%
Arg (R)  31	  4.8%
Asn (N)  38	  5.9%
Asp (D)  40	  6.2%
Cys (C)  14	  2.2%
Gln (Q)  26	  4.1%
Glu (E)  38	  5.9%
Gly (G)  29	  4.5%
His (H)  11	  1.7%
Ile (I)  51	  8.0%
Leu (L)  53	  8.3%
Lys (K)  52	  8.1%
Met (M)   9	  1.4%
Phe (F)  24	  3.8%
Pro (P)  31	  4.8%
Ser (S)  76	 11.9%
Thr (T)  39	  6.1%
Trp (W)   9	  1.4%
Tyr (Y)  13	  2.0%
Val (V)  36	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 83

Atomic composition:

Carbon      C	      3176
Hydrogen    H	      5076
Nitrogen    N	       880
Oxygen      O	       989
Sulfur      S	        23

Formula: C3176H5076N880O989S23
Total number of atoms: 10144

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69745
Abs 0.1% (=1 g/l)   0.967, assuming all pairs of Cys residues form cystines


Ext. coefficient    68870
Abs 0.1% (=1 g/l)   0.955, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.16
This classifies the protein as unstable.



Aliphatic index: 82.81

Grand average of hydropathicity (GRAVY): -0.487