ProtParam
User-provided sequence:
10 20 30 40 50 60
MSPSSTTTCT SLLEELQMIW DEIGESYNER DKMLLELEQE CLDIYNKKVE KTRKFRAELQ
70 80 90 100 110 120
RSLAQAEAEI ASLMSALGEK VSFAKKKEGS LKEQISSVKP VLEDLLMKKD RRRKELSETL
130 140 150 160 170 180
NQIAEITSNI AGNDYTVSSG SEVDESDLTQ RKLDELRADL QDLRNEKAVR LQKVNSYISA
190 200 210 220 230 240
VHELSEILSF DFSKALNSVH SSLTEFSKTH SKSISNDTLA RFTELVKSLK AEKHERLLKL
250 260 270 280 290 300
QGLGRSMQEL WNLMETPMDE RRRFDHCSSL LSSLPDDALK KGCLSLDIIR EAEDEVRRLN
310 320 330 340 350 360
SLKSSKMKEL VFKRQCELEE ICRGNHMDIN SDAARKSLVE LIESGDGDLS DILASIDGQI
370 380 390 400 410 420
EKAREEALSR KEILDKVDKW RHAKEEETWL DDYEKDENRF SAVRGAHKNL KRAEKARSLI
430 440 450 460 470 480
SKIPVFKQFN KKFWALLFSP KQLFKFSNSI IDYCENSTTH SMVDVLTTKV KAWEKERGVP
490 500 510 520 530 540
FLCDKQPLLQ TLEDDIVIRA QREEEKRQFR EQKRLQGQLA TEKEAKYGSK SAKKKPLGQS
550 560 570 580
LNTDNVTKTP IGRRIGNTPG RSVTSGGKDY RGNAVIPLNY VALQKDD
References and
documentation are available.
Number of amino acids: 587
Molecular weight: 67137.29
Theoretical pI: 7.55
Total number of negatively charged residues (Asp + Glu): 100
Total number of positively charged residues (Arg + Lys): 101
Atomic composition:
Carbon C 2922
Hydrogen H 4779
Nitrogen N 839
Oxygen O 929
Sulfur S 19
Formula: C
2922H
4779N
839O
929S
19
Total number of atoms: 9488
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 46910
Abs 0.1% (=1 g/l) 0.699, assuming all pairs of Cys residues form cystines
Ext. coefficient 46410
Abs 0.1% (=1 g/l) 0.691, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.02
This classifies the protein as unstable.
Aliphatic index: 83.92
Grand average of hydropathicity (GRAVY): -0.713