ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSPVIRRNLI ACLTLGLCVC LSFGPGPVES RTRPKNQVDR KLLTIYGSTP HVEALLGPGR 

        70         80         90        100        110        120 
PTPDTYKTLR SAFFRYEESR SLGHDLDLNS REIKANETIM KAKLKEFDEG LVTPHLFKPS 

       130        140        150        160        170        180 
QHIFDVLDGI RNTDLFKLLK KMPKGAVLHA HDTALCSTAA IINLTYYEHL WSCQQDGDLS 

       190        200        210        220        230        240 
ASALRFSKDK PQALSDCDWS LLSDVRAKYG ADKVDDYLAE RLTLYPTKKF EDNNAAWSTF 

       250        260        270        280        290        300 
MSIFNLLDGL VMYAPVWADY YYKALEEFYE DGVLYLEFRS VVPTLYDMDG TEFTPMDTVR 

       310        320        330        340        350        360 
IYVETLEKFK EAHPDFIGSR MIYAPIRYTN AEGVTGYIQT LKQIKEKYPE FVAGFDLVGQ 

       370        380        390        400        410        420 
EEMGRPLRDF VDELLSIPDD IDFYFHAGET NWFGSTVDEN LIDAILLGTK RIGHGFGLVK 

       430        440        450        460        470        480 
HPVVLDMLKK LNVAIEVNPI SNQVLQLVSD FRNHPCSHFF ADGYPVVISS DDPSFWKATP 

       490        500        510        520        530        540 
LTHDFYIAFL GIASQHSDLR LLKKLALNSI QYSSLTGDAQ FEALEKWQVK WDQFIADVND 

       550 
RSSNRGSPSQ RID 

References and documentation are available.

Number of amino acids: 553

Molecular weight: 62824.54

Theoretical pI: 5.39

Amino acid composition: 
Ala (A)  36	  6.5%
Arg (R)  26	  4.7%
Asn (N)  19	  3.4%
Asp (D)  46	  8.3%
Cys (C)   7	  1.3%
Gln (Q)  16	  2.9%
Glu (E)  30	  5.4%
Gly (G)  31	  5.6%
His (H)  15	  2.7%
Ile (I)  29	  5.2%
Leu (L)  63	 11.4%
Lys (K)  33	  6.0%
Met (M)  10	  1.8%
Phe (F)  31	  5.6%
Pro (P)  28	  5.1%
Ser (S)  38	  6.9%
Thr (T)  30	  5.4%
Trp (W)   8	  1.4%
Tyr (Y)  24	  4.3%
Val (V)  33	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2849
Hydrogen    H	      4375
Nitrogen    N	       737
Oxygen      O	       833
Sulfur      S	        17

Formula: C2849H4375N737O833S17
Total number of atoms: 8811

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    80135
Abs 0.1% (=1 g/l)   1.276, assuming all pairs of Cys residues form cystines


Ext. coefficient    79760
Abs 0.1% (=1 g/l)   1.270, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.03
This classifies the protein as stable.



Aliphatic index: 88.70

Grand average of hydropathicity (GRAVY): -0.240