ProtParam
User-provided sequence:
10 20 30 40 50 60
MSQAAKNVIV KLIVGAGQAA PSPPVGPALG SKGIKAIDFC KEFNARSANY QPGVPVPVLI
70 80 90 100 110 120
TIKPDRTFTF EMKSPPTGYL LLKALKMDKG HGQPNVGTML GSAPAKGPTR ALGELSLKHV
130 140 150
YEIAKIKKSD ERHSLLEMEG IVKSIVGVAK SMGIKVVP
References and
documentation are available.
Number of amino acids: 158
Molecular weight: 16669.77
Theoretical pI: 9.88
Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 751
Hydrogen H 1242
Nitrogen N 202
Oxygen O 209
Sulfur S 7
Formula: C
751H
1242N
202O
209S
7
Total number of atoms: 2411
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.268, assuming all pairs of Cys residues form cystines
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.268, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 26.68
This classifies the protein as stable.
Aliphatic index: 94.43
Grand average of hydropathicity (GRAVY): -0.001