ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQEEAESSI IYEEPIDIPL EDDDDEDELE EENSVPLSSQ ADQENAENES DDSVDNVVGS 

        70         80         90        100        110        120 
ETPRSVTGLS VDPRDVADEE DEDEEGEDED EDEDDNDVDN EDENDNDNAN ENENELGSSR 

       130        140        150        160        170        180 
DKRAPPAVQT SKRYKKYPKL DPAKAPPGKK VPLHLLEKRR LGRIKAAEEF AKTLKKIGIE 

       190        200        210        220        230        240 
KVETTTLPAT GLFQPLMLIN QKNYSSDYLK KDDQIFALRD RKFLRNNNTS QISSTNTPDV 

       250        260        270        280        290        300 
IDLKSLPHSE ASAAPLNDEI DLNDPTATIV IHPGSNSIKI GFPKDDHPVV VPNCVAVPKK 

       310        320        330        340        350        360 
WLDLENSEHV ENVCLQREQS EEFNNIKSEM EKNFRERMRY YKRKVPGNAH EQVVSFNENS 

       370        380        390        400        410        420 
KPEIISEKND PSPIEWIFDD SKLYYGSDAL RCVDEKFVIR KPFRGGSFNV KSPYYKSLAE 

       430        440        450        460        470        480 
LISDVTKLLE HALNSETLNV KPTKFNQYKV VLVIPDIFKK SHVETFIRVL LTELQFQAVA 

       490        500        510        520        530        540 
IIQESLATCY GAGISTSTCV VNIGAAETRI ACVDEGTVLE HSAITLDYGG DDITRLFALF 

       550        560        570        580        590        600 
LLQSDFPLQD WKIDSKHGWL LAERLKKNFT TFQDADVAVQ LYNFMNRSPN QPTEKYEFKL 

       610        620        630        640        650        660 
FDEVMLAPLA LFFPQIFKLI RTSSHKNSSL EFQLPESRDL FTNELNDWNS LSQFESKEGN 

       670        680        690        700        710        720 
LYCDLNDDLK ILNRILDAHN IIDQLQDKPE NYGNTLKENF APLEKAIVQS IANASITADV 

       730        740        750        760        770        780 
TRMNSFYSNI LIVGGSSKIP ALDFILTDRI NIWRPSLLSS ASFPQFYKKL TKEIKDLEGH 

       790        800        810        820        830        840 
YVNAPDKTED ENKQILQAQI KEKIVEELEE QHQNIEHQNG NEHIFPVSII PPPRDMNPAL 

       850        860        870        880 
IIWKGASVLA QIKLVEELFI TNSDWDVHGS RILQYKCIFT Y 

References and documentation are available.

Number of amino acids: 881

Molecular weight: 100209.08

Theoretical pI: 4.69

Amino acid composition: 
Ala (A)  50	  5.7%
Arg (R)  32	  3.6%
Asn (N)  62	  7.0%
Asp (D)  73	  8.3%
Cys (C)   8	  0.9%
Gln (Q)  35	  4.0%
Glu (E)  85	  9.6%
Gly (G)  29	  3.3%
His (H)  17	  1.9%
Ile (I)  63	  7.2%
Leu (L)  81	  9.2%
Lys (K)  65	  7.4%
Met (M)   8	  0.9%
Phe (F)  38	  4.3%
Pro (P)  48	  5.4%
Ser (S)  68	  7.7%
Thr (T)  38	  4.3%
Trp (W)   8	  0.9%
Tyr (Y)  23	  2.6%
Val (V)  50	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 158
Total number of positively charged residues (Arg + Lys): 97

Atomic composition:

Carbon      C	      4443
Hydrogen    H	      6951
Nitrogen    N	      1181
Oxygen      O	      1424
Sulfur      S	        16

Formula: C4443H6951N1181O1424S16
Total number of atoms: 14015

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    78770
Abs 0.1% (=1 g/l)   0.786, assuming all pairs of Cys residues form cystines


Ext. coefficient    78270
Abs 0.1% (=1 g/l)   0.781, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.85
This classifies the protein as unstable.



Aliphatic index: 85.88

Grand average of hydropathicity (GRAVY): -0.588