ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQEEKVDAK ATLKTEISNN KKNDKQELEL DELVGKLSIE GTPQVSQKLS KEEKHAHQLE 

        70         80         90        100        110        120 
ADSRSIFVGN ITPDVTPEQI EDHFKDCGQI KRITLLYDRN TGTPKGYGYI EFESPAYREK 

       130        140        150        160        170        180 
ALQLNGGELK GKKIAVSRKR TNIPGFNRHY NSQNQYFQQW QWNYPLMAYP NPDTFPYYPP 

       190        200        210        220        230        240 
YPPNQSPNQN FGYNKNNYYR SPYNNKNRTF QKKHFNSAKD STKNIRSTSQ KPVVMPSDNV 

       250 
KSSTQEKDSK 

References and documentation are available.

Number of amino acids: 250

Molecular weight: 28954.20

Theoretical pI: 9.31

Amino acid composition: 
Ala (A)   9	  3.6%
Arg (R)  10	  4.0%
Asn (N)  24	  9.6%
Asp (D)  12	  4.8%
Cys (C)   1	  0.4%
Gln (Q)  18	  7.2%
Glu (E)  17	  6.8%
Gly (G)  12	  4.8%
His (H)   5	  2.0%
Ile (I)  11	  4.4%
Leu (L)  13	  5.2%
Lys (K)  28	 11.2%
Met (M)   3	  1.2%
Phe (F)   9	  3.6%
Pro (P)  18	  7.2%
Ser (S)  20	  8.0%
Thr (T)  14	  5.6%
Trp (W)   2	  0.8%
Tyr (Y)  15	  6.0%
Val (V)   9	  3.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1279
Hydrogen    H	      1978
Nitrogen    N	       362
Oxygen      O	       400
Sulfur      S	         4

Formula: C1279H1978N362O400S4
Total number of atoms: 4023

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33350
Abs 0.1% (=1 g/l)   1.152, assuming all pairs of Cys residues form cystines


Ext. coefficient    33350
Abs 0.1% (=1 g/l)   1.152, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 58.47
This classifies the protein as unstable.



Aliphatic index: 51.48

Grand average of hydropathicity (GRAVY): -1.252