ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQEIRQNEK ISYRIEGPFF IIHLMNPDNL NALEGEDYIY LGELLELADR NRDVYFTIIQ 

        70         80         90        100        110        120 
SSGRFFSSGA DFKGIAKAQG DDTNKYPSET SKWVSNFVAR NVYVTDAFIK HSKVLICCLN 

       130        140        150        160        170        180 
GPAIGLSAAL VALCDIVYSI NDKVYLLYPF ANLGLITEGG TTVSLPLKFG TNTTYECLMF 

       190        200        210        220        230        240 
NKPFKYDIMC ENGFISKNFN MPSSNAEAFN AKVLEELREK VKGLYLPSCL GMKKLLKSNH 

       250        260        270        280 
IDAFNKANSV EVNESLKYWV DGEPLKRFRQ LGSKQRKHRL 

References and documentation are available.

Number of amino acids: 280

Molecular weight: 31698.42

Theoretical pI: 8.39

Amino acid composition: 
Ala (A)  17	  6.1%
Arg (R)  11	  3.9%
Asn (N)  22	  7.9%
Asp (D)  13	  4.6%
Cys (C)   6	  2.1%
Gln (Q)   6	  2.1%
Glu (E)  18	  6.4%
Gly (G)  18	  6.4%
His (H)   4	  1.4%
Ile (I)  18	  6.4%
Leu (L)  30	 10.7%
Lys (K)  23	  8.2%
Met (M)   6	  2.1%
Phe (F)  17	  6.1%
Pro (P)  10	  3.6%
Ser (S)  21	  7.5%
Thr (T)  10	  3.6%
Trp (W)   2	  0.7%
Tyr (Y)  13	  4.6%
Val (V)  15	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1431
Hydrogen    H	      2230
Nitrogen    N	       374
Oxygen      O	       415
Sulfur      S	        12

Formula: C1431H2230N374O415S12
Total number of atoms: 4462

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30745
Abs 0.1% (=1 g/l)   0.970, assuming all pairs of Cys residues form cystines


Ext. coefficient    30370
Abs 0.1% (=1 g/l)   0.958, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.96
This classifies the protein as stable.



Aliphatic index: 88.46

Grand average of hydropathicity (GRAVY): -0.220