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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQFFEAATP VAIPTNNTNG GSSDAGSAAT GGAPVVGTTA QPTINHRLLL SLKEAAKIIG

70 80 90 100 110 120
TKGSTISRIR AANAVKIGIS EKVPGCSDRI LSCAGNVINV ANAIGDIVDV LNKRNPENED

130 140 150 160 170 180
AAEGEAEEHY YFHFLNHILP APSKDEIRDL QQLEDIGYVR LIVANSHISS IIGKAGATIK

190 200 210 220 230 240
SLINKHGVKI VASKDFLPAS DERIIEIQGF PGSITNVLIE ISEIILSDVD VRFSTERSYF

250 260 270 280 290 300
PHLKKSSGEP TSPSTSSNTR IELKIPELYV GAIIGRGMNR IKNLKTFTKT NIVVERKDDD

310 320 330 340 350 360
DKDENFRKFI ITSKFPKNVK LAESMLLKNL NTEIEKRENY KRKLEAAEGD ATVVTERSDS

370 380
ASFLEEKEEP QENHDNKEEQ S


References and documentation are available.

Number of amino acids: 381 Molecular weight: 41683.02 Theoretical pI: 5.76
Amino acid composition: 
Ala (A) 31 8.1% Arg (R) 18 4.7% Asn (N) 26 6.8% Asp (D) 20 5.2% Cys (C) 2 0.5% Gln (Q) 7 1.8% Glu (E) 35 9.2% Gly (G) 24 6.3% His (H) 8 2.1% Ile (I) 39 10.2% Leu (L) 25 6.6% Lys (K) 30 7.9% Met (M) 3 0.8% Phe (F) 13 3.4% Pro (P) 16 4.2% Ser (S) 34 8.9% Thr (T) 22 5.8% Trp (W) 0 0.0% Tyr (Y) 6 1.6% Val (V) 22 5.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 55 Total number of positively charged residues (Arg + Lys): 48 Atomic composition: Carbon C 1827 Hydrogen H 2964 Nitrogen N 514 Oxygen O 587 Sulfur S 5 Formula: C1827H2964N514O587S5 Total number of atoms: 5897 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 9065 Abs 0.1% (=1 g/l) 0.217, assuming all pairs of Cys residues form cystines Ext. coefficient 8940 Abs 0.1% (=1 g/l) 0.214, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 45.72 This classifies the protein as unstable. Aliphatic index: 90.39 Grand average of hydropathicity (GRAVY): -0.397