ProtParam
User-provided sequence:
10 20 30 40 50 60
MSQFFEAATP VAIPTNNTNG GSSDAGSAAT GGAPVVGTTA QPTINHRLLL SLKEAAKIIG
70 80 90 100 110 120
TKGSTISRIR AANAVKIGIS EKVPGCSDRI LSCAGNVINV ANAIGDIVDV LNKRNPENED
130 140 150 160 170 180
AAEGEAEEHY YFHFLNHILP APSKDEIRDL QQLEDIGYVR LIVANSHISS IIGKAGATIK
190 200 210 220 230 240
SLINKHGVKI VASKDFLPAS DERIIEIQGF PGSITNVLIE ISEIILSDVD VRFSTERSYF
250 260 270 280 290 300
PHLKKSSGEP TSPSTSSNTR IELKIPELYV GAIIGRGMNR IKNLKTFTKT NIVVERKDDD
310 320 330 340 350 360
DKDENFRKFI ITSKFPKNVK LAESMLLKNL NTEIEKRENY KRKLEAAEGD ATVVTERSDS
370 380
ASFLEEKEEP QENHDNKEEQ S
References and
documentation are available.
Number of amino acids: 381
Molecular weight: 41683.02
Theoretical pI: 5.76
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 1827
Hydrogen H 2964
Nitrogen N 514
Oxygen O 587
Sulfur S 5
Formula: C
1827H
2964N
514O
587S
5
Total number of atoms: 5897
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 9065
Abs 0.1% (=1 g/l) 0.217, assuming all pairs of Cys residues form cystines
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.214, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.72
This classifies the protein as unstable.
Aliphatic index: 90.39
Grand average of hydropathicity (GRAVY): -0.397