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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQFVGFGVQ VELKDGKLIQ GKIAKATSKG LTLNDVQFGD GGKSQAFKVR ASRLKDLKVL

70 80 90 100 110 120
TVASQSGKRK QQRQQQQQND YNQNRGEHID WQDDDVSKIK QQEDFDFQRN LGMFNKKDVF

130 140 150 160 170 180
AQLKQNDDIL PENRLQGHNR KQTQLQQNNY QNDELVIPDA KKDSWNKISS RNEQSTHQSQ

190 200 210 220 230 240
PQQDAQDDLV LEDDEHEYDV DDIDDPKYLP ITQSLNITHL IHSATNSPSI NDKTKGTVIN

250 260 270 280 290 300
DKDQVLAKLG QMIISQSRSN STSLPAANKQ TTIRSKNTKQ NIPMATPVQL LEMESITSEF

310 320 330 340 350 360
FSINSAGLLE NFAVNASFFL KQKLGGRARL RLQNSNPEPL VVILASDSNR SGAKALALGR

370 380 390 400 410 420
HLCQTGHIRV ITLFTCSQNE LQDSMVKKQT DIYKKCGGKI VNSVSSLESA METLNSPVEI

430 440 450 460 470 480
VIDAMQGYDC TLSDLAGTSE VIESRIKSMI SWCNKQRGST KVWSLDIPNG FDAGSGMPDI

490 500 510 520 530 540
FFSDRIEATG IICSGWPLIA INNLIANLPS LEDAVLIDIG IPQGAYSQRT SLRKFQNCDL

550
FVTDGSLLLD L


References and documentation are available.

Number of amino acids: 551 Molecular weight: 61340.03 Theoretical pI: 5.99
Amino acid composition: 
Ala (A) 30 5.4% Arg (R) 22 4.0% Asn (N) 37 6.7% Asp (D) 45 8.2% Cys (C) 7 1.3% Gln (Q) 50 9.1% Glu (E) 22 4.0% Gly (G) 33 6.0% His (H) 8 1.5% Ile (I) 40 7.3% Leu (L) 52 9.4% Lys (K) 40 7.3% Met (M) 10 1.8% Phe (F) 19 3.4% Pro (P) 17 3.1% Ser (S) 52 9.4% Thr (T) 27 4.9% Trp (W) 5 0.9% Tyr (Y) 7 1.3% Val (V) 28 5.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 67 Total number of positively charged residues (Arg + Lys): 62 Atomic composition: Carbon C 2665 Hydrogen H 4293 Nitrogen N 765 Oxygen O 859 Sulfur S 17 Formula: C2665H4293N765O859S17 Total number of atoms: 8599 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 38305 Abs 0.1% (=1 g/l) 0.624, assuming all pairs of Cys residues form cystines Ext. coefficient 37930 Abs 0.1% (=1 g/l) 0.618, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 47.82 This classifies the protein as unstable. Aliphatic index: 85.30 Grand average of hydropathicity (GRAVY): -0.536