ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQGTLYANF RIRTWVPRGL VKALKLDVKV VTPDAAAEQF ARDFPLKKVP AFVGPKGYKL 

        70         80         90        100        110        120 
TEAMAINYYL VKLSQDDKMK TQLLGADDDL NAQAQIIRWQ SLANSDLCIQ IANTIVPLKG 

       130        140        150        160        170        180 
GAPYNKKSVD SAMDAVDKIV DIFENRLKNY TYLATENISL ADLVAASIFT RYFESLFGTE 

       190        200        210        220        230        240 
WRAQHPAIVR WFNTVRASPF LKDEYKDFKF ADKPLSPPQK KKEKKAPAAA PAASKKKEEA 

       250        260        270        280        290        300 
KPAATETETS SKKPKHPLEL LGKSTFVLDD WKRKYSNEDT RPVALPWFWE HYNPEEYSLW 

       310        320        330        340        350        360 
KVTYKYNDEL TLTFMSNNLV GGFFNRLSAS TKYMFGCLVV YGENNNNGIV GAVMVRGQDY 

       370        380        390        400        410 
VPAFDVAPDW ESYDYAKLDP TNDDDKEFIN NMWAWDKPVS VNGEPKEIVD GKVLK 

References and documentation are available.

Number of amino acids: 415

Molecular weight: 47087.72

Theoretical pI: 8.43

Amino acid composition: 
Ala (A)  41	  9.9%
Arg (R)  14	  3.4%
Asn (N)  24	  5.8%
Asp (D)  31	  7.5%
Cys (C)   2	  0.5%
Gln (Q)  11	  2.7%
Glu (E)  23	  5.5%
Gly (G)  18	  4.3%
His (H)   3	  0.7%
Ile (I)  15	  3.6%
Leu (L)  34	  8.2%
Lys (K)  43	 10.4%
Met (M)   8	  1.9%
Phe (F)  20	  4.8%
Pro (P)  25	  6.0%
Ser (S)  22	  5.3%
Thr (T)  21	  5.1%
Trp (W)  11	  2.7%
Tyr (Y)  19	  4.6%
Val (V)  30	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      2146
Hydrogen    H	      3298
Nitrogen    N	       552
Oxygen      O	       621
Sulfur      S	        10

Formula: C2146H3298N552O621S10
Total number of atoms: 6627

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    88935
Abs 0.1% (=1 g/l)   1.889, assuming all pairs of Cys residues form cystines


Ext. coefficient    88810
Abs 0.1% (=1 g/l)   1.886, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.73
This classifies the protein as stable.



Aliphatic index: 76.89

Grand average of hydropathicity (GRAVY): -0.466