ProtParam
User-provided sequence:
10 20 30 40 50 60
MSQHLLLLIL LSLLLLHKPI SATTIIQKFK EAPQFYNSAD CPLIDDSESD DDVVAKPIFC
70 80 90 100 110 120
SRRAVHVAMT LDAAYIRGSV AAVLSVLQHS SCPENIVFHF VASASADASS LRATISSSFP
130 140 150 160 170 180
YLDFTVYVFN VSSVSRLISS SIRSALDCPL NYARSYLADL LPPCVRRVVY LDSDLILVDD
190 200 210 220 230 240
IAKLAATDLG RDSVLAAPEY CNANFTSYFT STFWSNPTLS LTFADRKACY FNTGVMVIDL
250 260 270 280 290 300
SRWREGAYTS RIEEWMAMQK RMRIYELGSL PPFLLVFAGL IKPVNHRWNQ HGLGGDNFRG
310 320 330 340 350
LCRDLHPGPV SLLHWSGKGK PWARLDAGRP CPLDALWAPY DLLQTPFALD S
References and
documentation are available.
Number of amino acids: 351
Molecular weight: 39019.90
Theoretical pI: 6.49
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 1767
Hydrogen H 2737
Nitrogen N 467
Oxygen O 501
Sulfur S 15
Formula: C
1767H
2737N
467O
501S
15
Total number of atoms: 5487
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 58370
Abs 0.1% (=1 g/l) 1.496, assuming all pairs of Cys residues form cystines
Ext. coefficient 57870
Abs 0.1% (=1 g/l) 1.483, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.80
This classifies the protein as unstable.
Aliphatic index: 99.80
Grand average of hydropathicity (GRAVY): 0.145