ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQIVDFVED KDSRNDASIQ IIDGPSNVEI IALSESMDQD ECKRAHVSSA EMIPSSPQRK 

        70         80         90        100        110        120 
SVSNDVENVD LNKSIELSAP FFQDISISKL DDFSTTVNSI IDSSLRNENN AKGNAKKLLD 

       130        140        150        160        170        180 
DLISDEWSAD LESSGKKHNK SQYNLRDIAE KWGVQSLKNP EPIAVDCEYK TQGIGKTNSD 

       190        200        210        220        230        240 
ISDSPKSQIG AADILFDFPL SPVKHENPTE EKHNSIANEN SSPDNSLKPA GKQNHGEDGT 

       250        260        270        280        290        300 
SMAKRVYNKG EDEQEHLPKG KKRTIALSRT LINSTKLPDT VELNLSKFLD SSDSITTDVL 

       310        320        330        340        350        360 
STPAKGSNIV RTGSQPIFSN ANCFQEAKRS KTLTAEDPKC TKNTAREVSQ LENYIAYGQY 

       370        380        390        400        410        420 
YTREDSKNKI RHLLKENKNA FKRVNQIYRD NIKARSQMII EFSPSLLQLF KKGDSDLQQQ 

       430        440        450        460        470        480 
LAPAVVQSSY NDSMPLLRFL RKCDSIYDFS NDFYYPCDPK IVEENVLILY YDAQEFFEQY 

       490        500        510        520        530        540 
TSQKKELYRK IRFFSKNGKH VILILSDINK LKRAIFQLEN EKYKARVEQR LSGTEEALRP 

       550        560        570        580        590        600 
RSKKSSQVGK LGIKKFDLEQ RLRFIDREWH VKIHTVNSHM EFINSLPNLV SLIGKQRMDP 

       610        620        630        640        650        660 
AIRYMKYAHL NVKSAQDSTE TLKKTFHQIG RMPEMKANNV VSLYPSFQSL LEDIEKGRLQ 

       670        680        690 
SDNEGKYLMT EAVEKRLYKL FTCTDPNDTI E 

References and documentation are available.

Number of amino acids: 691

Molecular weight: 78763.73

Theoretical pI: 7.20

Amino acid composition: 
Ala (A)  35	  5.1%
Arg (R)  33	  4.8%
Asn (N)  47	  6.8%
Asp (D)  50	  7.2%
Cys (C)   7	  1.0%
Gln (Q)  34	  4.9%
Glu (E)  50	  7.2%
Gly (G)  24	  3.5%
His (H)  13	  1.9%
Ile (I)  48	  6.9%
Leu (L)  58	  8.4%
Lys (K)  67	  9.7%
Met (M)  12	  1.7%
Phe (F)  26	  3.8%
Pro (P)  28	  4.1%
Ser (S)  74	 10.7%
Thr (T)  29	  4.2%
Trp (W)   3	  0.4%
Tyr (Y)  22	  3.2%
Val (V)  31	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 100
Total number of positively charged residues (Arg + Lys): 100

Atomic composition:

Carbon      C	      3454
Hydrogen    H	      5513
Nitrogen    N	       967
Oxygen      O	      1098
Sulfur      S	        19

Formula: C3454H5513N967O1098S19
Total number of atoms: 11051

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49655
Abs 0.1% (=1 g/l)   0.630, assuming all pairs of Cys residues form cystines


Ext. coefficient    49280
Abs 0.1% (=1 g/l)   0.626, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.56
This classifies the protein as unstable.



Aliphatic index: 77.90

Grand average of hydropathicity (GRAVY): -0.734