ProtParam
User-provided sequence:
10 20 30 40 50 60
MSQKIGHSGL AFARLWHHVD VARDKRTLGR LASAIAITLI GRHKPVYHPS QDCGDYVVVT
70 80 90 100 110 120
NCQKIRVTGK KFEQKTYWSH SGRPGQLKLQ TMNKVVADKG FGEILKKAVS GMLPKNKLRK
130 140 150 160
QRLDRLKVFD GSENPYKQNI TAFAHEQSSI PEPLKESIFN QLK
References and
documentation are available.
Number of amino acids: 163
Molecular weight: 18463.42
Theoretical pI: 10.12
Total number of negatively charged residues (Asp + Glu): 13
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 824
Hydrogen H 1330
Nitrogen N 244
Oxygen O 228
Sulfur S 5
Formula: C
824H
1330N
244O
228S
5
Total number of atoms: 2631
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 17085
Abs 0.1% (=1 g/l) 0.925, assuming all pairs of Cys residues form cystines
Ext. coefficient 16960
Abs 0.1% (=1 g/l) 0.919, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.48
This classifies the protein as stable.
Aliphatic index: 80.74
Grand average of hydropathicity (GRAVY): -0.579