ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQLFNNSRS DTLFLGGEKI SGDDIRNQNV LATMAVANVV KSSLGPVGLD KMLVDDIGDF 

        70         80         90        100        110        120 
TVTNDGATIL SLLDVQHPAG KILVELAQQQ DREIGDGTTS VVIIASELLK RANELVKNKI 

       130        140        150        160        170        180 
HPTTIITGFR VALREAIRFI NEVLSTSVDT LGKETLINIA KTSMSSKIIG ADSDFFSNMV 

       190        200        210        220        230        240 
VDALLAVKTQ NSKGEIKYPV KAVNVLKAHG KSATESLLVP GYALNCTVAS QAMPKRIAGG 

       250        260        270        280        290        300 
NVKIACLDLN LQKARMAMGV QINIDDPEQL EQIRKREAGI VLERVKKIID AGAQVVLTTK 

       310        320        330        340        350        360 
GIDDLCLKEF VEAKIMGVRR CKKEDLRRIA RATGATLVSS MSNLEGEETF ESSYLGLCDE 

       370        380        390        400        410        420 
VVQAKFSDDE CILIKGTSKH SSSSIILRGA NDYSLDEMER SLHDSLSVVK RTLESGNVVP 

       430        440        450        460        470        480 
GGGCVEAALN IYLDNFATTV GSREQLAIAE FAAALLIIPK TLAVNAAKDS SELVAKLRSY 

       490        500        510        520        530        540 
HAASQMAKPE DVKRRSYRNY GLDLIRGKIV DEIHAGVLEP TISKVKSLKS ALEACVAILR 

       550 
IDTMITVDPE PPKEDPHDH 

References and documentation are available.

Number of amino acids: 559

Molecular weight: 60480.66

Theoretical pI: 6.08

Amino acid composition: 
Ala (A)  52	  9.3%
Arg (R)  27	  4.8%
Asn (N)  24	  4.3%
Asp (D)  37	  6.6%
Cys (C)   8	  1.4%
Gln (Q)  16	  2.9%
Glu (E)  36	  6.4%
Gly (G)  36	  6.4%
His (H)   9	  1.6%
Ile (I)  44	  7.9%
Leu (L)  58	 10.4%
Lys (K)  41	  7.3%
Met (M)  13	  2.3%
Phe (F)  12	  2.1%
Pro (P)  15	  2.7%
Ser (S)  45	  8.1%
Thr (T)  30	  5.4%
Trp (W)   0	  0.0%
Tyr (Y)   8	  1.4%
Val (V)  48	  8.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 73
Total number of positively charged residues (Arg + Lys): 68

Atomic composition:

Carbon      C	      2645
Hydrogen    H	      4389
Nitrogen    N	       739
Oxygen      O	       829
Sulfur      S	        21

Formula: C2645H4389N739O829S21
Total number of atoms: 8623

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12420
Abs 0.1% (=1 g/l)   0.205, assuming all pairs of Cys residues form cystines


Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.197, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.37
This classifies the protein as unstable.



Aliphatic index: 105.37

Grand average of hydropathicity (GRAVY): -0.035