ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQLFYQGDS DDELQEELTR QTTQASQSSK IKNEDEPDDS NHLNEVENED SKVLDDDAVL 

        70         80         90        100        110        120 
YPLIPNEPDD IETSKPNIND IRPVDIQLTL PLPFQQKVVE NSLITEDALI IMGKGLGLLD 

       130        140        150        160        170        180 
IVANLLHVLA TPTSINGQLK RALVLVLNAK PIDNVRIKEA LEELSWFSNT GKDDDDTAVE 

       190        200        210        220        230        240 
SDDELFERPF NVVTADSLSI EKRRKLYISG GILSITSRIL IVDLLSGIVH PNRVTGMLVL 

       250        260        270        280        290        300 
NADSLRHNSN ESFILEIYRS KNTWGFIKAF SEAPETFVME FSPLRTKMKE LRLKNVLLWP 

       310        320        330        340        350        360 
RFRVEVSSCL NATNKTSHNK VIEVKVSLTN SMSQIQFGLM ECLKKCIAEL SRKNPELALD 

       370        380        390        400        410        420 
WWNMENVLDI NFIRSIDSVM VPNWHRISYE SKQLVKDIRF LRHLLKMLVT SDAVDFFGEI 

       430        440        450        460        470        480 
QLSLDANKPS VSRKYSESPW LLVDEAQLVI SYAKKRIFYK NEYTLEENPK WEQLIHILHD 

       490        500        510        520        530        540 
ISHERMTNHL QGPTLVACSD NLTCLELAKV LNASNKKRGV RQVLLNKLKW YRKQREETKK 

       550        560        570        580        590        600 
LVKEVQSQDT FPENATLNVS STFSKEQVTT KRRRTRGASQ VAAVEKLRNA GTNVDMEVVF 

       610        620        630        640        650        660 
EDHKLSEEIK KGSGDDLDDG QEENAANDSK IFEIQEQENE ILIDDGDAEF DNGELEYVGD 

       670        680        690        700        710        720 
LPQHITTHFN KDLWAEHCNE YEYVDRQDEI LISTFKSLND NCSLQEMMPS YIIMFEPDIS 

       730        740        750        760        770        780 
FIRQIEVYKA IVKDLQPKVY FMYYGESIEE QSHLTAIKRE KDAFTKLIRE NANLSHHFET 

       790        800        810        820        830        840 
NEDLSHYKNL AERKLKLSKL RKSNTRNAGG QQGFHNLTQD VVIVDTREFN ASLPGLLYRY 

       850        860        870        880        890        900 
GIRVIPCMLT VGDYVITPDI CLERKSISDL IGSLQNNRLA NQCKKMLKYY AYPTLLIEFD 

       910        920        930        940        950        960 
EGQSFSLEPF SERRNYKNKD ISTVHPISSK LSQDEIQLKL AKLVLRFPTL KIIWSSSPLQ 

       970        980        990       1000       1010       1020 
TVNIILELKL GREQPDPSNA VILGTNKVRS DFNSTAKGLK DGDNESKFKR LLNVPGVSKI 

      1030       1040       1050       1060       1070       1080 
DYFNLRKKIK SFNKLQKLSW NEINELINDE DLTDRIYYFL RTEKEEQEQE STDENLESPG 

      1090       1100 
KTTDDNALHD HHNDVPEAPV 

References and documentation are available.

Number of amino acids: 1100

Molecular weight: 126370.23

Theoretical pI: 5.30

Amino acid composition: 
Ala (A)  46	  4.2%
Arg (R)  53	  4.8%
Asn (N)  76	  6.9%
Asp (D)  78	  7.1%
Cys (C)  10	  0.9%
Gln (Q)  45	  4.1%
Glu (E)  96	  8.7%
Gly (G)  38	  3.5%
His (H)  24	  2.2%
Ile (I)  75	  6.8%
Leu (L) 126	 11.5%
Lys (K)  85	  7.7%
Met (M)  18	  1.6%
Phe (F)  40	  3.6%
Pro (P)  40	  3.6%
Ser (S)  88	  8.0%
Thr (T)  54	  4.9%
Trp (W)  12	  1.1%
Tyr (Y)  29	  2.6%
Val (V)  67	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 174
Total number of positively charged residues (Arg + Lys): 138

Atomic composition:

Carbon      C	      5601
Hydrogen    H	      8913
Nitrogen    N	      1525
Oxygen      O	      1741
Sulfur      S	        28

Formula: C5601H8913N1525O1741S28
Total number of atoms: 17808

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   109835
Abs 0.1% (=1 g/l)   0.869, assuming all pairs of Cys residues form cystines


Ext. coefficient   109210
Abs 0.1% (=1 g/l)   0.864, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.38
This classifies the protein as unstable.



Aliphatic index: 93.11

Grand average of hydropathicity (GRAVY): -0.512