ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQPTKNKKK EHGTDSKSSR MTRTLVNHIL FERILPILPV ESNLSTYSEV EEYSSFISCR 

        70         80         90        100        110        120 
SVLINVTVSR DANAMVEGTL ELIESLLQGH EIISDKGSSD VIESILIILR LLSDALEYNW 

       130        140        150        160        170        180 
QNQESLHYND ISTHVEHDQE QKYRPKLNSI LPDYSSTHSN GNKHFFHQSK PQALIPELAS 

       190        200        210        220        230        240 
KLLESCAKLK FNTRTLQILQ NMISHVHGNI LTTLSSSILP RHKSYLTRHN HPSHCKMIDS 

       250        260        270        280        290        300 
TLGHILRFVA ASNPSEYFEF IRKSVQVPVT QTHTHSHSHS HSLPSSVYNS IVPHFDLFSF 

       310        320        330        340        350        360 
IYLSKHNFKK YLELIKNLSV TLRKTIYHCL LLHYSAKAIM FWIMARPAEY YELFNLLKDN 

       370        380        390        400        410        420 
NNEHSKSLNT LNHTLFEEIH STFNVNSMIT TNQNAHQGSS SPSSSSPSSP PSSSSSDNNN 

       430        440        450        460        470        480 
QNIIAKSLSR QLSHHQSYIQ QQSERKLHSS WTTNSQSSTS LSSSTSNSTT TDFSTHTQPG 

       490        500        510        520        530        540 
EYDPSLPDTP TMSNITISAS SLLSQTPTPT TQLQQRLNSA AAAAAAAASP SNSTPTGYTA 

       550        560        570        580        590        600 
EQQSRASYDA HKTGHTGKDY DEHFLSVTRL DNVLELYTHF DDTEVLPHTS VLKFLTTLTM 

       610        620        630        640        650        660 
FDIDLFNELN ATSFKYIPDC TMHRPKERTS SFNNTAHETG SEKTSGIKHI TQGLKKLTSL 

       670        680        690        700        710        720 
PSSTKKTVKF VKMLLRNLNG NQAVSDVALL DTMRALLSFF TMTSAVFLVD RNLPSVLFAK 

       730        740        750        760        770        780 
RLIPIMGTNL SVGQDWNSKI NNSLMVCLKK NSTTFVQLQL IFFSSAIQFD HELLLARLSI 

       790        800        810        820        830        840 
DTMANNLNMQ KLCLYTEGFR IFFDIPSKKE LRKAIAVKIS KFFKTLFSII ADILLQEFPY 

       850        860        870        880        890        900 
FDEQITDIVA SILDGTIINE YGTKKHFKGS SPSLCSTTRS RSGSTSQSSM TPVSPLGLDT 

       910        920        930        940        950        960 
DICPMNTLSL VGSSTSRNSD NVNSLNSSPK NLSSDPYLSH LVAPRARHAL GGPSSIIRNK 

       970        980        990       1000       1010       1020 
IPTTLTSPPG TEKSSPVQRP QTESISATPM AITNSTPLSS AAFGIRSPLQ KIRTRRYSDE 

      1030       1040       1050       1060       1070       1080 
SLGKFMKSTN NYIQEHLIPK DLNEATLQDA RRIMINIFSI FKRPNSYFII PHNINSNLQW 

      1090       1100       1110       1120       1130       1140 
VSQDFRNIMK PIFVAIVSPD VDLQNTAQSF MDTLLSNVIT YGESDENISI EGYHLLCSYT 

      1150       1160       1170       1180       1190       1200 
VTLFAMGLFD LKINNEKRQI LLDITVKFMK VRSHLAGIAE ASHHMEYISD SEKLTFPLIM 

      1210       1220       1230       1240       1250       1260 
GTVGRALFVS LYSSQQKIEK TLKIAYTEYL SAINFHERNI DDADKTWVHN IEFVEAMCHD 

      1270       1280       1290       1300       1310       1320 
NYTTSGSIAF QRRTRNNILR FATIPNAILL DSMRMIYKKW HTYTHSKSLE KQERNDFRNF 

      1330       1340       1350       1360       1370       1380 
AGILASLSGI LFINKKILQE MYPYLLDTVS ELKKNIDSFI SKQCQWLNYP DLLTRENSRD 

      1390       1400       1410       1420       1430       1440 
ILSVELHPLS FNLLFNNLRL KLKELACSDL SIPENESSYV LLEQIIKMLR TILGRDDDNY 

      1450       1460       1470       1480       1490       1500 
VMMLFSTEIV DLIDLLTDEI KKIPAYCPKY LKAIIQMTKM FSALQHSEVN LGVKNHFHVK 

      1510       1520       1530       1540       1550       1560 
NKWLRQITDW FQVSIAREYD FENLSKPLKE MDLVKRDMDI LYIDTAIEAS TAIAYLTRHT 

      1570       1580       1590       1600       1610       1620 
FLEIPPAASD PELSRSRSVI FGFYFNILMK GLEKSSDRDN YPVFLRHKMS VLNDNVILSL 

      1630       1640       1650       1660       1670       1680 
TNLSNTNVDA SLQFTLPMGY SGNRNIRNAF LEVFINIVTN YRTYTAKTDL GKLEAADKFL 

      1690       1700       1710       1720       1730       1740 
RYTIEHPQLS SFGAAVCPAS DIDAYAAGLI NAFETRNATH IVVAQLIKNE IEKSSRPTDI 

      1750       1760       1770       1780       1790       1800 
LRRNSCATRS LSMLARSKGN EYLIRTLQPL LKKIIQNRDF FEIEKLKPED SDAERQIELF 

      1810       1820       1830       1840       1850       1860 
VKYMNELLES ISNSVSYFPP PLFYICQNIY KVACEKFPDH AIIAAGSFVF LRFFCPALVS 

      1870       1880       1890       1900       1910       1920 
PDSENIIDIS HLSEKRTFIS LAKVIQNIAN GSENFSRWPA LCSQKDFLKE CSDRIFRFLA 

      1930       1940       1950       1960       1970       1980 
ELCRTDRTID IQVRTDPTPI AFDYQFLHSF VYLYGLEVRR NVLNEAKHDD GDIDGDDFYK 

      1990       2000       2010       2020       2030       2040 
TTFLLIDDVL GQLGQPKMEF SNEIPIYIRE HMDDYPELYE FMNRHAFRNI ETSTAYSPSV 

      2050       2060       2070       2080       2090       2100 
HESTSSEGIP IITLTMSNFS DRHVDIDTVA YKFLQIYARI WTTKHCLIID CTEFDEGGLD 

      2110       2120       2130       2140       2150       2160 
MRKFISLVMG LLPEVAPKNC IGCYYFNVNE TFMDNYGKCL DKDNVYVSSK IPHYFINSNS 

      2170       2180       2190       2200       2210       2220 
DEGLMKSVGI TGQGLKVLQD IRVSLHDITL YDEKRNRFTP VSLKIGDIYF QVLHETPRQY 

      2230       2240       2250       2260       2270       2280 
KIRDMGTLFD VKFNDVYEIS RIFEVHVSSI TGVAAEFTVT FQDERRLIFS SPKYLEIVKM 

      2290       2300       2310       2320       2330       2340 
FYYAQIRLES EYEMDNNSST SSPNSNNKDK QQKERTKLLC HLLLVSLIGL FDESKKMKNS 

      2350       2360       2370       2380       2390       2400 
SYNLIAATEA SFGLNFGSHF HRSPEVYVPE DTTTFLGVIG KSLAESNPEL TAYMFIYVLE 

      2410       2420       2430       2440       2450       2460 
ALKNNVIPHV YIPHTICGLS YWIPNLYQHV YLADDEEGPE NISHIFRILI RLSVRETDFK 

      2470       2480       2490       2500       2510       2520 
AVYMQYVWLL LLDDGRLTDI IVDEVINHAL ERDSENRDWK KTISLLTVLP TTEVANNIIQ 

      2530       2540       2550       2560       2570       2580 
KILAKIRSFL PSLKLEAMTQ SWSELTILVK ISIHVFFETS LLVQMYLPEI LFIVSLLIDV 

      2590       2600       2610       2620       2630       2640 
GPRELRSSLH QLLMNVCHSL AINSALPQDH RNNLDEISDI FAHQKVKFMF GFSEDKGRIL 

      2650       2660       2670       2680       2690       2700 
QIFSASSFAS KFNILDFFIN NILLLMEYSS TYEANVWKTR YKKYVLESVF TSNSFLSARS 

      2710       2720       2730       2740       2750       2760 
IMIVGIMGKS YITEGLCKAM LIETMKVIAE PKITDEHLFL AISHIFTYSK IVEGLDPNLD 

      2770       2780       2790       2800       2810       2820 
LMKHLFWFST LFLESRHPII FEGALLFVSN CIRRLYMAQF ENESETSLIS TLLKGRKFAH 

      2830       2840       2850       2860       2870       2880 
TFLSKIENLS GIVWNEDNFT HILIFIINKG LSNPFIKSTA FDFLKMMFRN SYFEHQINQK 

      2890       2900       2910       2920       2930       2940 
SDHYLCYMFL LYFVLNCNQF EELLGDVDFE GEMVNIENKN TIPKILLEWL SSDNENANIT 

      2950       2960       2970       2980       2990       3000 
LYQGAILFKC SVTDEPSRFR FALIIRHLLT KKPICALRFY SVIRNEIRKI SAFEQNSDCV 

      3010       3020       3030       3040       3050       3060 
PLAFDILNLL VTHSESNSLE KLHEESIERL TKRGLSIVTS SGIFAKNSDM MIPLDVKPED 

      3070 
IYERKRIMTM ILSRMSCSA 

References and documentation are available.

Number of amino acids: 3079

Molecular weight: 351668.54

Theoretical pI: 6.59

Amino acid composition: 
Ala (A) 147	  4.8%
Arg (R) 136	  4.4%
Asn (N) 189	  6.1%
Asp (D) 157	  5.1%
Cys (C)  38	  1.2%
Gln (Q)  97	  3.2%
Glu (E) 179	  5.8%
Gly (G)  93	  3.0%
His (H) 101	  3.3%
Ile (I) 258	  8.4%
Leu (L) 339	 11.0%
Lys (K) 179	  5.8%
Met (M)  79	  2.6%
Phe (F) 171	  5.6%
Pro (P) 117	  3.8%
Ser (S) 332	 10.8%
Thr (T) 197	  6.4%
Trp (W)  20	  0.6%
Tyr (Y) 109	  3.5%
Val (V) 141	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 336
Total number of positively charged residues (Arg + Lys): 315

Atomic composition:

Carbon      C	     15792
Hydrogen    H	     24766
Nitrogen    N	      4174
Oxygen      O	      4676
Sulfur      S	       117

Formula: C15792H24766N4174O4676S117
Total number of atoms: 49525

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   274785
Abs 0.1% (=1 g/l)   0.781, assuming all pairs of Cys residues form cystines


Ext. coefficient   272410
Abs 0.1% (=1 g/l)   0.775, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.04
This classifies the protein as unstable.



Aliphatic index: 93.67

Grand average of hydropathicity (GRAVY): -0.184