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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQPTPIITT KSAAKPKPKI FNLFRVCFIS LLLIAAVEYF KYGTRINYEW FHCTPIKEPQ

70 80 90 100 110 120
SGSVIKLWAR GGPSCDKRGE YKTIVKRITR DYEPNDEHLS FCIIENDNVP PVHYPIHEDK

130 140 150
GEPGYVAYVG YDTDSELVQE LCADSTIYHM


References and documentation are available.

Number of amino acids: 150 Molecular weight: 17206.69 Theoretical pI: 6.20
Amino acid composition: 
Ala (A) 7 4.7% Arg (R) 6 4.0% Asn (N) 5 3.3% Asp (D) 8 5.3% Cys (C) 5 3.3% Gln (Q) 3 2.0% Glu (E) 11 7.3% Gly (G) 8 5.3% His (H) 5 3.3% Ile (I) 14 9.3% Leu (L) 8 5.3% Lys (K) 11 7.3% Met (M) 2 1.3% Phe (F) 6 4.0% Pro (P) 12 8.0% Ser (S) 9 6.0% Thr (T) 9 6.0% Trp (W) 2 1.3% Tyr (Y) 10 6.7% Val (V) 9 6.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 19 Total number of positively charged residues (Arg + Lys): 17 Atomic composition: Carbon C 782 Hydrogen H 1193 Nitrogen N 199 Oxygen O 225 Sulfur S 7 Formula: C782H1193N199O225S7 Total number of atoms: 2406 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 26150 Abs 0.1% (=1 g/l) 1.520, assuming all pairs of Cys residues form cystines Ext. coefficient 25900 Abs 0.1% (=1 g/l) 1.505, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 40.56 This classifies the protein as unstable. Aliphatic index: 79.27 Grand average of hydropathicity (GRAVY): -0.361