ProtParam
User-provided sequence:
10 20 30 40 50 60
MSQQDNVKAA AEGVANLHLD EATGEMVSKS ELKKRIKQRQ VEAKKAAKKA AAQPKPASKK
70 80 90 100 110 120
KTDLFADLDP SQYFETRSRQ IQELRKTHEP NPYPHKFHVS ISNPEFLAKY AHLKKGETLP
130 140 150 160 170 180
EEKVSIAGRI HAKRESGSKL KFYVLHGDGV EVQLMSQLQD YCDPDSYEKD HDLLKRGDIV
190 200 210 220 230 240
GVEGYVGRTQ PKKGGEGEVS VFVSRVQLLT PCLHMLPADH FGFKDQETRY RKRYLDLIMN
250 260 270 280 290 300
KDARNRFITR SEIIRYIRRF LDQRKFIEVE TPMMNVIAGG ATAKPFITHH NDLDMDMYMR
310 320 330 340 350 360
IAPELFLKQL VVGGLDRVYE IGRQFRNEGI DMTHNPEFTT CEFYQAYADV YDLMDMTELM
370 380 390 400 410 420
FSEMVKEITG SYIIKYHPDP ADPAKELELN FSRPWKRINM IEELEKVFNV KFPSGDQLHT
430 440 450 460 470 480
AETGEFLKKI LVDNKLECPP PLTNARMLDK LVGELEDTCI NPTFIFGHPQ MMSPLAKYSR
490 500 510 520 530 540
DQPGLCERFE VFVATKEICN AYTELNDPFD QRARFEEQAR QKDQGDDEAQ LVDETFCNAL
550 560 570 580 590
EYGLPPTGGW GCGIDRLAMF LTDSNTIREV LLFPTLKPDV LREEVKKEEE N
References and
documentation are available.
Number of amino acids: 591
Molecular weight: 67958.55
Theoretical pI: 5.78
Total number of negatively charged residues (Asp + Glu): 96
Total number of positively charged residues (Arg + Lys): 83
Atomic composition:
Carbon C 3018
Hydrogen H 4739
Nitrogen N 825
Oxygen O 903
Sulfur S 29
Formula: C
3018H
4739N
825O
903S
29
Total number of atoms: 9514
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41300
Abs 0.1% (=1 g/l) 0.608, assuming all pairs of Cys residues form cystines
Ext. coefficient 40800
Abs 0.1% (=1 g/l) 0.600, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.12
This classifies the protein as unstable.
Aliphatic index: 75.25
Grand average of hydropathicity (GRAVY): -0.602