ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQQTPQESE QTTAKEQDLD QESVLSNIDF NTDLNHNLNL SEYCISSDAG TEKMDSDEEK 

        70         80         90        100        110        120 
SLANLPELKY APKLSSLVKQ ETLTESLKRP HEDEKEAIDE AKKMKVPGEN EDESKEEEKS 

       130        140        150        160        170        180 
QELEEAIDSK EKSTDARDEQ GDEGDNEEEN NEEDNENENE HTAPPALVMP SPIEMEEQRM 

       190        200        210        220        230        240 
TALKEITDIE YKFAQLRQKL YDNQLVRLQT ELQMCLEGSH PELQVYYSKI AAIRDYKLHR 

       250        260        270        280        290        300 
AYQRQKYELS CINTETIATR TFIHQDFHKK VTDLRARLLN RTTQTWYDIN KERRDMDIVI 

       310        320        330        340        350        360 
PDVNYHVPIK LDNKTLSCIT GYASAAQLCY PGEPVAEDLA CESIEYRYRA NPVDKLEVIV 

       370        380        390        400        410        420 
DRMRLNNEIS DLEGLRKYFH SFPGAPELNP LRDSEINDDF HQWAQCDRHT GPHTTSFCYS 

References and documentation are available.

Number of amino acids: 420

Molecular weight: 48671.68

Theoretical pI: 4.64

Amino acid composition: 
Ala (A)  25	  6.0%
Arg (R)  21	  5.0%
Asn (N)  24	  5.7%
Asp (D)  34	  8.1%
Cys (C)   8	  1.9%
Gln (Q)  23	  5.5%
Glu (E)  55	 13.1%
Gly (G)  10	  2.4%
His (H)  12	  2.9%
Ile (I)  21	  5.0%
Leu (L)  37	  8.8%
Lys (K)  28	  6.7%
Met (M)   9	  2.1%
Phe (F)   8	  1.9%
Pro (P)  19	  4.5%
Ser (S)  28	  6.7%
Thr (T)  25	  6.0%
Trp (W)   2	  0.5%
Tyr (Y)  17	  4.0%
Val (V)  14	  3.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 89
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      2096
Hydrogen    H	      3290
Nitrogen    N	       584
Oxygen      O	       716
Sulfur      S	        17

Formula: C2096H3290N584O716S17
Total number of atoms: 6703

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36830
Abs 0.1% (=1 g/l)   0.757, assuming all pairs of Cys residues form cystines


Ext. coefficient    36330
Abs 0.1% (=1 g/l)   0.746, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.00
This classifies the protein as unstable.



Aliphatic index: 69.48

Grand average of hydropathicity (GRAVY): -0.995