ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQRKLQQDI DKLLKKVKEG IEDFDDIYEK FQSTDPSNSS HREKLESDLK REIKKLQKHR 

        70         80         90        100        110        120 
DQIKTWLSKE DVKDKQSVLM TNRRLIENGM ERFKSVEKLM KTKQFSKEAL TNPDIIKDPK 

       130        140        150        160        170        180 
ELKKRDQVLF IHDCLDELQK QLEQYEAQEN EEQTERHEFH IANLENILKK LQNNEMDPEP 

       190        200        210        220        230        240 
VEEFQDDIKY YVENNDDPDF IEYDTIYEDM GCEIQPSSSN NEAPKEGNNQ TSLSSIRSSK 

       250        260        270        280        290        300 
KQERSPKKKA PQRDVSISDR ATTPIAPGVE SASQSISSTP TPVSTDTPLH TVKDDSIKFD 

       310        320        330        340        350        360 
NSTLGTPTTH VSMKKKESEN DSEQQLNFPP DRTDEIRKTI QHDVETNAAF QNPLFNDELK 

       370        380        390        400        410        420 
YWLDSKRYLM QPLQEMSPKM VSQLESSLLN CPDSLDADSP CLYTKPLSLP HPTSIFFPNE 

       430        440        450        460        470        480 
PIRFVYPYDV PLNLTNNEND TDNKFGKDSK AKSKKDDDIY SRTSLARIFM KFDLDTLFFI 

       490        500        510        520        530        540 
FYHYQGSYEQ FLAARELFKN RNWLFNKVDR CWYYKEIEKL PPGMGKSEEE SWRYFDYKKS 

       550        560 
WLARRCGNDF VYNEEDFEKL 

References and documentation are available.

Number of amino acids: 560

Molecular weight: 65854.61

Theoretical pI: 5.20

Amino acid composition: 
Ala (A)  16	  2.9%
Arg (R)  25	  4.5%
Asn (N)  33	  5.9%
Asp (D)  51	  9.1%
Cys (C)   6	  1.1%
Gln (Q)  30	  5.4%
Glu (E)  53	  9.5%
Gly (G)  11	  2.0%
His (H)  10	  1.8%
Ile (I)  28	  5.0%
Leu (L)  46	  8.2%
Lys (K)  59	 10.5%
Met (M)  12	  2.1%
Phe (F)  27	  4.8%
Pro (P)  32	  5.7%
Ser (S)  49	  8.8%
Thr (T)  28	  5.0%
Trp (W)   6	  1.1%
Tyr (Y)  20	  3.6%
Val (V)  18	  3.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 84

Atomic composition:

Carbon      C	      2905
Hydrogen    H	      4519
Nitrogen    N	       783
Oxygen      O	       929
Sulfur      S	        18

Formula: C2905H4519N783O929S18
Total number of atoms: 9154

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63175
Abs 0.1% (=1 g/l)   0.959, assuming all pairs of Cys residues form cystines


Ext. coefficient    62800
Abs 0.1% (=1 g/l)   0.954, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.17
This classifies the protein as unstable.



Aliphatic index: 63.71

Grand average of hydropathicity (GRAVY): -1.047