ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQRSFGSEI GNMTAATNSN DIADVSLATP LKSTEQMEKS ENGTAVSRLP KPLAIEVYSK 

        70         80         90        100        110        120 
VHNSNLSEDD LLDEIEDLID EKFFAGEKLM SSGKEYTVVS SEKRGGLTLY TMEDGTKIGH 

       130        140        150        160        170        180 
RDLRRKKGLS VEEIKKIAIE DAEFVDEKQW KVREELLAEN PIREIKKYAP IFSANRKSTP 

       190        200        210        220        230        240 
KTAQLSVAAE ADDSDVQEVS MDAESGAEAA NKSAMKTPRG APRASGGPVI LSSRRLQEKQ 

       250        260        270        280        290        300 
KEKDEKEKEK LEKKQKEQEE KKQKKEEEKA KKLKEKEEKL KEKEEKAARK AEKKEKNNGT 

       310        320        330        340        350        360 
MDKFLKKDTG SPSSKNAPLF SPSKWGEKRI AVGVKKMEDA WKRRDLELYN EACSWCEKNL 

       370        380        390        400        410        420 
SGNQRSTFEN PIFKFSVQKL VDKAKDRAHM KGMKWAQKAE FKAEMSEKRK ELYQKFEPKI 

       430        440        450        460        470        480 
KAWFNEDIAL DDLLVTCENL ELLDAKRVNC DEELLKCLEI SQFFVSMRKI LLWNENITAE 

       490        500        510        520        530        540 
QLRDDLHGGF DGFKRSTYKM IANLLETALQ EKEHEKAAHC NARLSEFPIN EHTISELIRA 

       550        560        570        580        590        600 
FFIGSTETSF KRDGKRAARG AHDDDDEEDG DEEEMDSREK VVEIEEKPEE NVENGAAENG 

       610        620        630        640        650        660 
EIGDDEEENS ESELQKQRIL ALFADSCHIY ELPAGAQLEV LCAMKEVVHG LPIIREWFLR 

       670        680        690        700        710        720 
DANSEKLTEH KKEANRIRNE MEQFHQQLQD FPPIPEITES MTRTQTREAE QSLRRREKLE 

       730        740        750        760        770        780 
NQLDTLKIEL EENREATARE VDDLERIFRV VHIGNDRHLR KYYWFAYSSD AAIWVQDFGT 

       790        800        810        820        830        840 
TSYEKWVRDC SEKGFMDVES SDVENRPEYE DLPLTSSQSS ETWYKLDTEP AIRQLMTQLK 

       850        860        870        880        890        900 
TNGKREKLLK KYLRNNMDDI ISSILRKEKK QKKDDGEEED EEASEEDEAS AAAENGEEKM 

       910        920        930        940        950        960 
ETEQNETAAA AAAETQEAEK NARRFTGRFG SLKRTMSELL NDWKQSGISK IVDSQVFEAR 

       970        980        990       1000       1010       1020 
MLEANTLDEM KRLVTELVTS IPVECVIEKF PQNVAIAKKF LNFSTILLEK WKNYEFSDFF 

      1030       1040       1050       1060       1070       1080 
RAKCRIFNYF LLFSHKTSKF LEKLNQTMKI GAKFGICAIF QKPKDVRLLP KKKECFSHLK 

      1090       1100       1110       1120       1130       1140 
MPRFCRRVQE ASNASCLHML LAYFDARIDQ QRTLPELSCQ VCRRKTGTER KLMCKQCSTV 

      1150       1160       1170       1180       1190       1200 
FHYGCHRPTI SRALFEEEGF KEGWWCAKCT KEDRRRQLSE AKEDLRQKEE GGGDEEDHHG 

      1210       1220       1230       1240       1250       1260 
SGESDDEDEE DIVEEETRGR SAKRKANAAM RDVLEFEGVL RQTPAPPPPK RQKTVVVPEV 

      1270       1280       1290       1300       1310       1320 
LELFNSIERA NPRLYKTLQQ IPGQSRSTRN AQHENRSLPD IEQDLDVYTS AEQLHEHLSQ 

      1330       1340       1350       1360       1370 
FFRHARGYIE THNPRKFSKF SCPFKIFFHP PATSFGIFLK FFIMAFDHFI 

References and documentation are available.

Number of amino acids: 1370

Molecular weight: 158293.51

Theoretical pI: 5.67

Amino acid composition: 
Ala (A) 103	  7.5%
Arg (R)  89	  6.5%
Asn (N)  55	  4.0%
Asp (D)  80	  5.8%
Cys (C)  23	  1.7%
Gln (Q)  55	  4.0%
Glu (E) 182	 13.3%
Gly (G)  57	  4.2%
His (H)  28	  2.0%
Ile (I)  63	  4.6%
Leu (L) 109	  8.0%
Lys (K) 142	 10.4%
Met (M)  31	  2.3%
Phe (F)  65	  4.7%
Pro (P)  44	  3.2%
Ser (S)  93	  6.8%
Thr (T)  61	  4.5%
Trp (W)  16	  1.2%
Tyr (Y)  22	  1.6%
Val (V)  52	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 262
Total number of positively charged residues (Arg + Lys): 231

Atomic composition:

Carbon      C	      6920
Hydrogen    H	     10997
Nitrogen    N	      1961
Oxygen      O	      2181
Sulfur      S	        54

Formula: C6920H10997N1961O2181S54
Total number of atoms: 22113

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   122155
Abs 0.1% (=1 g/l)   0.772, assuming all pairs of Cys residues form cystines


Ext. coefficient   120780
Abs 0.1% (=1 g/l)   0.763, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.87
This classifies the protein as unstable.



Aliphatic index: 67.49

Grand average of hydropathicity (GRAVY): -0.875