ProtParam
User-provided sequence:
10 20 30 40 50 60
MSQRSSQHIV GIHYAVGPKI GEGSFGVIFE GENILHSCQA QTGSKRDSSI IMANEPVAIK
70 80 90 100 110 120
FEPRHSDAPQ LRDEFRAYRI LNGCVGIPHA YYFGQEGMHN ILIIDLLGPS LEDLFEWCGR
130 140 150 160 170 180
KFSVKTTCMV AKQMIDRVRA IHDHDLIYRD IKPDNFLISQ YQRISPEGKV IKSCASSSNN
190 200 210 220 230 240
DPNLIYMVDF GMAKQYRDPR TKQHIPYRER KSLSGTARYM SINTHFGREQ SRRDDLESLG
250 260 270 280 290 300
HVFFYFLRGS LPWQGLKAPN NKLKYEKIGM TKQKLNPDDL LLNNAIPYQF ATYLKYARSL
310 320 330 340 350 360
KFDEDPDYDY LISLMDDALR LNDLKDDGHY DWMDLNGGKG WNIKINRRAN LHGYGNPNPR
370 380 390 400 410 420
VNGNTARNNV NTNSKTRNTT PVATPKQQAQ NSYNKDNSKS RISSNPQSFT KQQHVLKKIE
430 440 450 460 470 480
PNSKYIPETH SNLQRPIKSQ SQTYDSISHT QNSPFVPYSS SKANPKRSNN EHNLPNHYTN
490 500 510 520
LANKNINYQS QRNYEQENDA YSDDENDTFC SKIYKYCCCC FCCC
References and
documentation are available.
Number of amino acids: 524
Molecular weight: 60260.61
Theoretical pI: 8.97
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 68
Atomic composition:
Carbon C 2641
Hydrogen H 4087
Nitrogen N 769
Oxygen O 805
Sulfur S 24
Formula: C
2641H
4087N
769O
805S
24
Total number of atoms: 8326
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 65960
Abs 0.1% (=1 g/l) 1.095, assuming all pairs of Cys residues form cystines
Ext. coefficient 65210
Abs 0.1% (=1 g/l) 1.082, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.38
This classifies the protein as unstable.
Aliphatic index: 65.53
Grand average of hydropathicity (GRAVY): -0.834