ProtParam
User-provided sequence:
10 20 30 40 50 60
MSQSISLKGS TRTVTEFFEY SINSILYQRG VYPAEDFVTV KKYDLTLLKT HDDELKDYIR
70 80 90 100 110 120
KILLQVHRWL LGGKCNQLVL CIVDKDEGEV VERWSFNVQH ISGNSNGQDD VVDLNTTQSQ
130 140 150 160 170 180
IRALIRQITS SVTFLPELTK EGGYTFTVLA YTDADAKVPL EWADSNSKEI PDGEVVQFKT
190
FSTNDHKVGA QVSYKY
References and
documentation are available.
Number of amino acids: 196
Molecular weight: 22284.08
Theoretical pI: 5.24
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 21
Atomic composition:
Carbon C 998
Hydrogen H 1557
Nitrogen N 261
Oxygen O 311
Sulfur S 3
Formula: C
998H
1557N
261O
311S
3
Total number of atoms: 3130
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30035
Abs 0.1% (=1 g/l) 1.348, assuming all pairs of Cys residues form cystines
Ext. coefficient 29910
Abs 0.1% (=1 g/l) 1.342, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.42
This classifies the protein as stable.
Aliphatic index: 89.39
Grand average of hydropathicity (GRAVY): -0.355